24, 25-Dihydroxy VD3

Cat. No.: HY-76915 Purity: 98.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

24, 25-Dihydroxy VD3 is a compound closely related to 1,25-dihydroxyvitamin D3 and is the active form of vitamin D3.

For research use only. We do not sell to patients.

24, 25-Dihydroxy VD3 Chemical Structure

CAS No. : 40013-87-4

Size	Price	Stock	Quantity
1 mg	USD 1056	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 3960	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

24, 25-Dihydroxy VD3 is a compound closely related to 1,25-dihydroxyvitamin D3 and is the active form of vitamin D3^[1].

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

24, 25-Dihydroxy VD3 (24R,25(OH)2D3) (0.002-202 nM) inhibits the increase in intracellular Ca²⁺ concentration in a dose-dependent manner, with an EC₅₀ of 4.9 nM and a maximum inhibition rate of 60% in Atlantic cod of both sexes intestinal cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

416.64

Formula

C₂₇H₄₄O₃

CAS No.

40013-87-4

Appearance

Solid

Color

White to off-white

SMILES

C=C1/C(C[C@@H](O)CC1)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CCC(O)C(C)(C)O)CC3

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (120.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4002 mL	12.0008 mL	24.0015 mL
5 mM	0.4800 mL	2.4002 mL	4.8003 mL
10 mM	0.2400 mL	1.2001 mL	2.4002 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.60%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (251 KB) RP-HPLC (249 KB)

Handling Instructions (2659 KB)

References

[1]. D Larsson, et al. Antagonistic effects of 24R,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3 on L-type Ca2+ channels and Na+/Ca2+ exchange in enterocytes from Atlantic cod (Gadus morhua). J Mol Endocrinol. 2002 Feb;28(1):53-68. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4002 mL	12.0008 mL	24.0015 mL	60.0038 mL
	5 mM	0.4800 mL	2.4002 mL	4.8003 mL	12.0008 mL
	10 mM	0.2400 mL	1.2001 mL	2.4002 mL	6.0004 mL
	15 mM	0.1600 mL	0.8001 mL	1.6001 mL	4.0003 mL
	20 mM	0.1200 mL	0.6000 mL	1.2001 mL	3.0002 mL
	25 mM	0.0960 mL	0.4800 mL	0.9601 mL	2.4002 mL
	30 mM	0.0800 mL	0.4000 mL	0.8001 mL	2.0001 mL
	40 mM	0.0600 mL	0.3000 mL	0.6000 mL	1.5001 mL
	50 mM	0.0480 mL	0.2400 mL	0.4800 mL	1.2001 mL
	60 mM	0.0400 mL	0.2000 mL	0.4000 mL	1.0001 mL
	80 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7500 mL
	100 mM	0.0240 mL	0.1200 mL	0.2400 mL	0.6000 mL