3,4,7,8-Tetramethyl-1,10-phenanthroline
Based on 1 Customer Validation
3,4,7,8-Tetramethyl-1,10-phenanthroline (TMPhen) is an organic molecule commonly used as a ligand or catalyst. It has a wide range of applications in different fields, such as organometallic chemical reactions, electrochemical detection, and organic optoelectronic devices. Due to its excellent performance in fluorescent probes, biosensors and photocatalytic reactions, it has been widely used in research in the fields of chemistry and life sciences.
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 1660-93-1
- Formula: C16H16N2
- Molecular Weight:236.31
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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HY-W004544
o-Phenanthroline -
HY-W004567
5-Chloro-1,10-phenanthroline -
HY-W034953
Bathocuproine disulfonate disodium -
HY-Y1841
o-Phenanthroline monohydrate -
HY-W034551
DOTA-tri(t-butyl ester) -
HY-W004586
4'-Chloro-2,2':6',2''-terpyridine -
HY-W053583
Tetraxetan -
HY-W011060
Cryptand 2.2.2 -
HY-Y1841R
o-Phenanthroline monohydrate (Standard) -
HY-W004583
2,9-Dichloro-1,10-phenanthroline -
HY-111024
2,2,5,7,8-Pentamethyl-6-Chromanol -
HY-12692
DO3A tert-Butyl ester
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-7 | EC50 |
0.48 μM
Compound: 8
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Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis
Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis
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[PMID: 22957890] |
| COS-7 | EC50 |
1 μM
Compound: 8
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Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis
Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis
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[PMID: 22957890] |
| COS-7 | EC50 |
1.5 μM
Compound: 8
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Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis
Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis
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[PMID: 22957890] |
| COS-7 | IC50 |
171 μM
Compound: 8
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Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs
Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs
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[PMID: 22957890] |
In Vitro
3,4,7,8-Tetramethyl-1,10-phenanthroline is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1660-93-1
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Appearance Solid
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Molecular Weight 236.31
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Formula C16H16N2
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Color White to off-white
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SMILES
CC1=C(C)C=NC2=C(N=CC(C)=C3C)C3=CC=C21
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Synonyms
TMPhen; 3,4,7,8-Tetramethyl-1,10-phenanthroline
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
In Vitro:
Ethanol : 50 mg/mL (211.59 mM; warming and heat to 60°C)
DMSO : 2 mg/mL (8.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.58 mg/mL (10.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (246 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 4.2317 mL | 21.1586 mL | 42.3173 mL | 105.7932 mL |
| 5 mM | 0.8463 mL | 4.2317 mL | 8.4635 mL | 21.1586 mL | |
| Ethanol | 10 mM | 0.4232 mL | 2.1159 mL | 4.2317 mL | 10.5793 mL |
| 15 mM | 0.2821 mL | 1.4106 mL | 2.8212 mL | 7.0529 mL | |
| 20 mM | 0.2116 mL | 1.0579 mL | 2.1159 mL | 5.2897 mL | |
| 25 mM | 0.1693 mL | 0.8463 mL | 1.6927 mL | 4.2317 mL | |
| 30 mM | 0.1411 mL | 0.7053 mL | 1.4106 mL | 3.5264 mL | |
| 40 mM | 0.1058 mL | 0.5290 mL | 1.0579 mL | 2.6448 mL | |
| 50 mM | 0.0846 mL | 0.4232 mL | 0.8463 mL | 2.1159 mL | |
| 60 mM | 0.0705 mL | 0.3526 mL | 0.7053 mL | 1.7632 mL | |
| 80 mM | 0.0529 mL | 0.2645 mL | 0.5290 mL | 1.3224 mL | |
| 100 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0579 mL |
3,4,7,8-Tetramethyl-1,10-phenanthroline Related Classifications
HY-34515 Related Classifications
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