3-Hydroxypyridine
Based on 1 Customer Validation
3-Hydroxypyridine is a compound that can be isolated from bamboo grass. As an endogenous photosensitizer present in human skin, 3-hydroxypyridine can mediate oxidative stress, proliferation inhibition and apoptosis of skin cells through UVA/UVB excitation.
For research use only. We do not sell to patients.
- Purity : 99.42%
- CAS No.: 109-00-2
- Formula: C5H5NO
- Molecular Weight:95.10
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-B1745
PyridoxylamineEndogenous Metabolite -
HY-Y1129R
3-Hydroxypyridine (Standard)Standard of 3-Hydroxypyridine -
HY-Y0191
α-PyridoneAntibacterial Agent -
HY-W007690
Pyridin-4-olSynthetic Intermediate -
HY-Y0198
2,6-DiaminopyridineIntermediate -
HY-W025817
Citrazinic acidFluorescent Compound -
HY-Y0198R
2,6-Diaminopyridine (Standard)Standard Of 2,6-Diaminopyridine -
HY-Y0191R
α-Pyridone (Standard) -
HY-20159R
Nicotinonitrile (Standard) -
HY-75128R
2-Amino-5-methylpyridine (Standard)
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| MIA PaCa-2 | IC50 |
56480 nM
Compound: S34, r1c8
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Cytotoxicity against human MIA PaCa2 cells after 4 hrs by MTT assay
Cytotoxicity against human MIA PaCa2 cells after 4 hrs by MTT assay
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[PMID: 17944451] |
| MIA PaCa-2 | IC50 |
73100 nM
Compound: S34, r1c8
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Cytotoxicity against human MIA PaCa2 cells after 72 hrs by MTT assay
Cytotoxicity against human MIA PaCa2 cells after 72 hrs by MTT assay
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[PMID: 17944451] |
| MRC5 | IC50 |
>500 μM
Compound: 2c
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Cytostatic activity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytostatic activity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29289891] |
| T98G | IC50 |
>500 μM
Compound: 2c
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Cytostatic activity against human T98G cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytostatic activity against human T98G cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29289891] |
| U-118-MG | IC50 |
>500 μM
Compound: 2c
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Cytostatic activity against human U118MG cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytostatic activity against human U118MG cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29289891] |
| U-87MG ATCC | IC50 |
>500 μM
Compound: 2c
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Cytostatic activity against human U87MG cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytostatic activity against human U87MG cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29289891] |
In Vitro
3-Hydroxypyridine (100 μM; 3 days) inhibits proliferation and induces cell cycle arrest and apoptosis in HaCaT keratinocytes after solar simulated light irradiation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 109-00-2
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Appearance Solid
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Molecular Weight 95.10
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Formula C5H5NO
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Color White to off-white
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SMILES
OC1=CC=CN=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 110 mg/mL (1156.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.23 mg/mL (44.48 mM); Clear solution
This protocol yields a clear solution of ≥ 4.23 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (42.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.75 mg/mL (28.92 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 10.5152 mL | 52.5762 mL | 105.1525 mL | 262.8812 mL |
| 5 mM | 2.1030 mL | 10.5152 mL | 21.0305 mL | 52.5762 mL | |
| 10 mM | 1.0515 mL | 5.2576 mL | 10.5152 mL | 26.2881 mL | |
| 15 mM | 0.7010 mL | 3.5051 mL | 7.0102 mL | 17.5254 mL | |
| 20 mM | 0.5258 mL | 2.6288 mL | 5.2576 mL | 13.1441 mL | |
| 25 mM | 0.4206 mL | 2.1030 mL | 4.2061 mL | 10.5152 mL | |
| 30 mM | 0.3505 mL | 1.7525 mL | 3.5051 mL | 8.7627 mL | |
| 40 mM | 0.2629 mL | 1.3144 mL | 2.6288 mL | 6.5720 mL | |
| 50 mM | 0.2103 mL | 1.0515 mL | 2.1030 mL | 5.2576 mL | |
| 60 mM | 0.1753 mL | 0.8763 mL | 1.7525 mL | 4.3814 mL | |
| 80 mM | 0.1314 mL | 0.6572 mL | 1.3144 mL | 3.2860 mL | |
| 100 mM | 0.1052 mL | 0.5258 mL | 1.0515 mL | 2.6288 mL |
3-Hydroxypyridine Related Classifications
HY-Y1129 Related Classifications
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