Cat. No.: HY-W010934

3β,5α,6β-Trihydroxycholestane

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3β,5α,6β-Trihydroxycholestane is a steroid that occurs naturally in the body and is also found in certain foods. It belongs to a class of compounds known as cholestanes, which are closely related to the better known cholesterol. This particular compound is formed from cholesterol through a series of enzymatic reactions in the liver and other organs. It has been studied for its potential health benefits, including its ability to reduce inflammation and oxidative stress in the body. Some research suggests that it may also play a role in regulating blood sugar levels and improving insulin sensitivity. Despite these potential benefits, 3β,5α,6β-Trihydroxycholestane is not widely used as a supplement or medicine due to its relatively low content, focus on natural resources and limited research. However, researchers continue to investigate its potential uses and effects on human health.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 1253-84-5
Formula: C₂₇H₄₈O₃
Molecular Weight:420.67

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

3β,5α,6β-Trihydroxycholestane Chemical Structure

CAS No.: 1253-84-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	17.9 μM Compound: 2	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
A549	IC₅₀	17.9 μM Compound: 4	Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
ARPE-19	IC₅₀	19.1 μM Compound: 2	Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
ARPE-19	IC₅₀	19.1 μM Compound: 4	Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
BJ	IC₅₀	18.4 μM Compound: 2	Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
BJ	IC₅₀	18.6 μM Compound: 4	Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HEK293	EC₅₀	1.7 μM Compound: 8	Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy	[PMID: 24928400]
HepG2	IC₅₀	10.9 μM Compound: 2	Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HepG2	IC₅₀	10.9 μM Compound: 4	Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HMEC-1	IC₅₀	15.2 μM Compound: 4	Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	10.3 μM Compound: 4	Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	12.9 μM Compound: 2	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HT-29	IC₅₀	12.9 μM Compound: 4	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
LAMA-84	IC₅₀	5 μM Compound: 2	Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
LAMA-84	IC₅₀	5 μM Compound: 4	Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
MCF7	IC₅₀	13.6 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
MCF7	IC₅₀	13.6 μM Compound: 4	Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
PC-3	IC₅₀	17.4 μM Compound: 2	Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
PC-3	IC₅₀	17.4 μM Compound: 4	Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
SH-SY5Y	IC₅₀	17.2 μM Compound: 2	Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]

In Vitro

3β,5α,6β-Trihydroxycholestane is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1253-84-5
Appearance Solid
Molecular Weight 420.67
Formula C₂₇H₄₈O₃
Color White to off-white
SMILES

O[C@H]1CC[C@]2(C)[C@@]3([H])CC[C@]4(C)[C@@H]([C@@H](CCCC(C)C)C)CC[C@@]4([H])[C@]3([H])C[C@@H](O)[C@@]2(O)C1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (237.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3772 mL

11.8858 mL

23.7716 mL

5 mM

0.4754 mL

2.3772 mL

4.7543 mL

10 mM

0.2377 mL

1.1886 mL

2.3772 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (248 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3772 mL	11.8858 mL	23.7716 mL	59.4290 mL
	5 mM	0.4754 mL	2.3772 mL	4.7543 mL	11.8858 mL
	10 mM	0.2377 mL	1.1886 mL	2.3772 mL	5.9429 mL
	15 mM	0.1585 mL	0.7924 mL	1.5848 mL	3.9619 mL
	20 mM	0.1189 mL	0.5943 mL	1.1886 mL	2.9715 mL
	25 mM	0.0951 mL	0.4754 mL	0.9509 mL	2.3772 mL
	30 mM	0.0792 mL	0.3962 mL	0.7924 mL	1.9810 mL
	40 mM	0.0594 mL	0.2971 mL	0.5943 mL	1.4857 mL
	50 mM	0.0475 mL	0.2377 mL	0.4754 mL	1.1886 mL
	60 mM	0.0396 mL	0.1981 mL	0.3962 mL	0.9905 mL
	80 mM	0.0297 mL	0.1486 mL	0.2971 mL	0.7429 mL
	100 mM	0.0238 mL	0.1189 mL	0.2377 mL	0.5943 mL

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3β,5α,6β-Trihydroxycholestane

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

Help & FAQs

Complete Stock Solution Preparation Table

3β,5α,6β-Trihydroxycholestane Related Classifications

HY-W010934 Related Classifications