2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Glycosidase
  4. 4-Methylumbelliferyl-α-D-Glucopyranoside

4-Methylumbelliferyl-α-D-Glucopyranoside  (Synonyms: 4-Methylumbelliferyl-α-D-Glucose; 4-Methylumbelliferyl-α-D-Glucoside; 4-MU-α-D-Glucopyranoside)

Cat. No.: HY-137873 Purity: 99.99%
COA
SDS
Handling Instructions Technical Support

4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.

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4-Methylumbelliferyl-α-D-Glucopyranoside

4-Methylumbelliferyl-α-D-Glucopyranoside 화학구조

CAS No. : 17833-43-1

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

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4-Methylumbelliferyl-α-D-Glucopyranoside Related Antibodies

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  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.

In Vitro

Guide (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
GAA enzyme assay[2]
1. Preparation of 4-Methylumbelliferyl-α-D-Glucopyranoside working solution
1) Prepare 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside working solution.
2. Experimental steps
1) Harvest fibroblasts and HEK293T cells by trypsinization and disrupt by freeze-thawing (3 times).
2) Mix the cell homogenate (30-40 μg protein) with 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside in 0.2 acetate buffer (pH 4.0) and incubate at 37° for 60 min.
3) The reaction is stopped with 1.9 mL of glycine carbonate buffer (pH 10.7) and fluorescence is read on a Turner Biosystems Modulus 9200 fluorometer (360 nm excitation, 450 nm emission).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl-α-D-Glucopyranoside Related Antibodies
분자량

338.31

화학식

C16H18O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)C2=CC=C(O[C@@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C=C2O1
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

용액&용해도
In Vitro: 

DMSO : 25 mg/mL (73.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9559 mL 14.7793 mL 29.5587 mL
5 mM 0.5912 mL 2.9559 mL 5.9117 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9559 mL 14.7793 mL 29.5587 mL 73.8967 mL
5 mM 0.5912 mL 2.9559 mL 5.9117 mL 14.7793 mL
10 mM 0.2956 mL 1.4779 mL 2.9559 mL 7.3897 mL
15 mM 0.1971 mL 0.9853 mL 1.9706 mL 4.9264 mL
20 mM 0.1478 mL 0.7390 mL 1.4779 mL 3.6948 mL
25 mM 0.1182 mL 0.5912 mL 1.1823 mL 2.9559 mL
30 mM 0.0985 mL 0.4926 mL 0.9853 mL 2.4632 mL
40 mM 0.0739 mL 0.3695 mL 0.7390 mL 1.8474 mL
50 mM 0.0591 mL 0.2956 mL 0.5912 mL 1.4779 mL
60 mM 0.0493 mL 0.2463 mL 0.4926 mL 1.2316 mL
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4-Methylumbelliferyl-α-D-Glucopyranoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl-α-D-Glucopyranoside17833-43-14-Methylumbelliferyl-α-D-Glucose 4-Methylumbelliferyl-α-D-Glucoside 4-MU-α-D-GlucopyranosideFluorescent DyeGlycosidaseInhibitorinhibitorinhibit

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상품명:
4-Methylumbelliferyl-α-D-Glucopyranoside
Cat. No.:
HY-137873
수량:
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