4-Nitrophenyl α-D-galactopyranoside (Synonyms: PNP-alpha-D-Gal; PNP-α-D-Gal; 4-ニトロフェニルα-D-ガラクトピラノシド)

Name: 4-Nitrophenyl α-D-galactopyranoside
Brand: MedChemExpress
SKU: HY-W039911
Rating: 4.5 (0 reviews)

製品番号: HY-W039911 純度: 98.70%: Data Sheet SDS COA 取扱説明書
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4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when 4-Nitrophenyl α-D-galactopyranoside is used as substrates.

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4-Nitrophenyl α-D-galactopyranoside 構造式

CAS 番号 : 7493-95-0

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 44	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 44	在庫あり	Estimated Time of Arrival: December 31
Solid
100 mg	$40	在庫あり	Estimated Time of Arrival: December 31
250 mg	$65	在庫あり	Estimated Time of Arrival: December 31
500 mg		お問い合わせ
1 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 0 publication(s) in Google Scholar

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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	25 mM Compound: p-Nitrophenyl-alpha-Gal	Inhibition of Cryptosporidium parvum recombinant Galactose/N-acetylgalactosamine-specific lectin binding to Caco2A cells Inhibition of Cryptosporidium parvum recombinant Galactose/N-acetylgalactosamine-specific lectin binding to Caco2A cells	[PMID: 17905738]

体外実験

Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0^[1].
The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

301.25

分子式

C₁₂H₁₅NO₈

CAS 番号

7493-95-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O)[C@@H](O)[C@@H](OC2=CC=C([N+]([O-])=O)C=C2)O[C@@H]1CO

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

H₂O : 16.67 mg/mL (55.34 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3195 mL	16.5975 mL	33.1950 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (82.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 98.70%

Select Batch:

データシート (273 KB) SDS (393 KB)

COA (250 KB) HNMR (263 KB) LCMS (96 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Okamoto-Nakazato A. Implications of the galactosidase activity of yieldin in the regulatory mechanism of yield threshold that is fundamental to cell wall extension. Physiol Plant. 2018 Jun;163(2):259-266. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.3195 mL	16.5975 mL	33.1950 mL	82.9876 mL
	5 mM	0.6639 mL	3.3195 mL	6.6390 mL	16.5975 mL
	10 mM	0.3320 mL	1.6598 mL	3.3195 mL	8.2988 mL
	15 mM	0.2213 mL	1.1065 mL	2.2130 mL	5.5325 mL
	20 mM	0.1660 mL	0.8299 mL	1.6598 mL	4.1494 mL
	25 mM	0.1328 mL	0.6639 mL	1.3278 mL	3.3195 mL
	30 mM	0.1107 mL	0.5533 mL	1.1065 mL	2.7663 mL
	40 mM	0.0830 mL	0.4149 mL	0.8299 mL	2.0747 mL
	50 mM	0.0664 mL	0.3320 mL	0.6639 mL	1.6598 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

4-Nitrophenyl α-D-galactopyranoside Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl α-D-galactopyranoside7493-95-0PNP-alpha-D-Gal PNP-α-D-GalBiochemical Assay Reagents4-nitrophenylα-galactosidaseglycopyranosideacidic-pHyieldinartificialsubstrateInhibitorinhibitorinhibit

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4-Nitrophenyl α-D-galactopyranoside (Synonyms: PNP-alpha-D-Gal; PNP-α-D-Gal; 4-ニトロフェニルα-D-ガラクトピラノシド)

カスタマーレビュー

4-Nitrophenyl α-D-galactopyranoside 関連抗体

おすすめ

•関連小分子:

•関連タンパク質:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

4-Nitrophenyl α-D-galactopyranoside 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

4-Nitrophenyl α-D-galactopyranoside Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report