(4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate
(4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate is a polyacetylene found in rhizomes of Atractylodes chinensis. (4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate inhibits RANKL-induced osteoclast differentiation. (4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate can be used for the research of osteoporosis.
For research use only. We do not sell to patients.
- CAS No.: 13894-76-3
- Formula: C16H18O3
- Molecular Weight:258.31
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
(4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate (Compound 5) (10 μM; 72 h) shows no cytotoxicity in bone marrow-derived macrophages (BMMs)[1].
(4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate (10 μM; 6 days) inhibits RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Bone marrow-derived macrophages (BMMs)
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Concentration:10 μM
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Incubation Time:72 h
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Result:Showed no cytotoxicity.
Chemical Information
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CAS No. 13894-76-3
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Molecular Weight 258.31
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Formula C16H18O3
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SMILES
C/C=C/C#CC#C/C=C/C=C/C(O)CCOC(C)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Osteoclast differentiation from monocyte/macrophage precursors
Osteoclast differentiation is an in vitro induction assay in which monocyte/macrophage-lineage precursors are exposed to macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL), generating multinucleated osteoclasts that are commonly identified by tartrate-resistant acid phosphatase (TRAP) staining and functionally confirmed by resorption pits on dentin, bone, or mineralized substrates. M-CSF supports survival and expansion of osteoclast precursors, while RANKL binding to RANK drives osteoclast commitment, fusion, maturation, and resorptive function; osteoprotegerin inhibits this pathway by binding RANKL and preventing RANK activation. The main readouts are the number of TRAP-positive multinucleated cells, formation of F-actin rings, and resorbed surface area; TRAP-positive multinucleated cells indicate osteoclast differentiation, whereas pit formation on dentin, bone, or mineralized coating indicates functional bone-resorbing activity.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(4E,6E,12E)-3-Hydroxy-4,6,12-tetradecatriene-8,10-diyn-1-yl acetate Related Classifications
HY-N18265 Related Classifications
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