5α,6α-Epoxysitosterol
5α,6α-Epoxysitosterol is a minor β-sitosterol oxidation product and 5,6-epoxy thermo-oxidation product of sitosterol .
For research use only. We do not sell to patients.
- CAS No.: 53034-66-5
- Formula: C29H50O2
- Molecular Weight:430.72
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
5α,6α-Epoxysitosterol (5,6α-epoxysitosterol) is a minor thermal oxidation product generated from pure β-sitosterol at 180 °C[1].
5α,6α-Epoxysitosterol is a minor thermal oxidation product formed from β-sitosterol in corn oil, lard and olive oil at 180 °C[1].
5α,6α-Epoxysitosterol is a product formed by heating natural sitosterol in rapeseed oil[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 53034-66-5
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Appearance Solid
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Molecular Weight 430.72
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Formula C29H50O2
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Color White to off-white
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SMILES
C[C@]12[C@@]3([C@@](O3)(C[C@@]4([C@@]1(CC[C@@]5(C)[C@]4(CC[C@@]5([C@@H](CC[C@H](C(C)C)CC)C)[H])[H])[H])[H])[H])C[C@@H](O)CC2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
Ethanol : 10 mg/mL (23.22 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.32 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 1 mg/mL (2.32 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
References
[1]. Xu G, Guan L, Sun J, et al.. Oxidation of cholesterol and beta-sitosterol and prevention by natural antioxidants. Journal of agricultural and food chemistry. 2009 Oct 14;57(19):9284-92. [Content Brief]
[2]. Lampi AM, Juntunen L, Toivo J, et al.. Determination of thermo-oxidation products of plant sterols. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2002 Sep 25;777(1-2):83-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.3217 mL | 11.6085 mL | 23.2169 mL | 58.0423 mL |
| 5 mM | 0.4643 mL | 2.3217 mL | 4.6434 mL | 11.6085 mL | |
| 10 mM | 0.2322 mL | 1.1608 mL | 2.3217 mL | 5.8042 mL | |
| 15 mM | 0.1548 mL | 0.7739 mL | 1.5478 mL | 3.8695 mL | |
| 20 mM | 0.1161 mL | 0.5804 mL | 1.1608 mL | 2.9021 mL |
5α,6α-Epoxysitosterol Related Classifications
HY-N20654 Related Classifications
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