5-Hydroxymethyl-2'-deoxyuridine
Based on 1 Customer Validation
5-Hydroxymethyl-2'-deoxyuridine is a nucleoside analog. 5-Hydroxymethyl-2'-deoxyuridine inhibits the replication of multiple human leukemia cell lines with IC50 values of 1.7-5.8 μM. 5-Hydroxymethyl-2'-deoxyuridine prolongs the survival of mice carrying L1210 leukemia. 5-Hydroxymethyl-2'-deoxyuridine can be used for the research of cell replication and leukemia.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 5116-24-5
- Formula: C10H14N2O6
- Molecular Weight:258.23
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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HY-L025
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CEM-SS | CC50 |
120 μM
Compound: 1c
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Cytotoxicity against human CEM-SS cells by MTT assay
Cytotoxicity against human CEM-SS cells by MTT assay
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[PMID: 20112915] |
| CEM-SS | IC50 |
30 μM
Compound: 1c
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Antiviral activity against HIV1 LAI in human CEM-SS cells assessed as RT activity after 5 days by single passage assay
Antiviral activity against HIV1 LAI in human CEM-SS cells assessed as RT activity after 5 days by single passage assay
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[PMID: 20112915] |
| L5178Y | IC50 |
0.002 μM
Compound: HmdUrd
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In vitro growth inhibition of L5178Y-Parental murine leukemia cells
In vitro growth inhibition of L5178Y-Parental murine leukemia cells
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[PMID: 11101356] |
| L5178Y | IC50 |
11 μM
Compound: HmdUrd
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In vitro growth inhibition of FdUrd resistant L5178Y-Resistant murine leukemia cells
In vitro growth inhibition of FdUrd resistant L5178Y-Resistant murine leukemia cells
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[PMID: 11101356] |
| L5178Y | IC50 |
8.6 μM
Compound: HmdUrd
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In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd.
In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd.
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[PMID: 11101356] |
| MT4 | CC50 |
130 μM
Compound: 1c
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Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
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[PMID: 20112915] |
| MT4 | IC50 |
>130 μM
Compound: 1c
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Antiviral activity against HIV1 3B in human MT4 cells assessed as RT activity after 5 days by single passage assay
Antiviral activity against HIV1 3B in human MT4 cells assessed as RT activity after 5 days by single passage assay
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[PMID: 20112915] |
5-Hydroxymethyl-2'-deoxyuridine (0-10 μM) inhibits the replication of Sarcoma 180 cells and Ehrlich ascites carcinoma cells with ED50 values of 8.5 and 4 μM, respectively[1]. 5-Hydroxymethyl-2'-deoxyuridine inhibits herpes simplex virus type 1 (HSV-1) pyrimidine 2’-deoxyribonucleoside kinase with a Ki value of 3.5 μM[1]. 5-Hydroxymethyl-2'-deoxyuridine inhibits the replication of multiple human leukemia cell lines with IC50 values of 1.7-5.8 μM[2]. 5-Hydroxymethyl-2'-deoxyuridine (10-100 μM) shows dose-dependent toxicity against a human acute promyelocytic leukemia cell line[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5116-24-5
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Appearance Solid
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Molecular Weight 258.23
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Formula C10H14N2O6
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Color White to off-white
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SMILES
O[C@H]1C[C@H](N2C(NC(C(CO)=C2)=O)=O)O[C@@H]1CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 116.67 mg/mL (451.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Shiau GT, et al. Synthesis and biological activities of 5-(hydroxymethyl, azidomethyl, or aminomethyl)-2'-deoxyuridine and related 5'-substituted analogues. J Med Chem. 1980 Feb;23(2):127-33. [Content Brief]
[2]. Kahilainen LI, et al. 5-Hydroxymethyl-2'-deoxyuridine. Cytotoxicity and DNA incorporation studied by using a novel [2-14C]-derivative with normal and leukemic human hematopoietic cells. Acta Chem Scand B. 1985;39(6):477-84. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8725 mL | 19.3626 mL | 38.7252 mL | 96.8129 mL |
| 5 mM | 0.7745 mL | 3.8725 mL | 7.7450 mL | 19.3626 mL | |
| 10 mM | 0.3873 mL | 1.9363 mL | 3.8725 mL | 9.6813 mL | |
| 15 mM | 0.2582 mL | 1.2908 mL | 2.5817 mL | 6.4542 mL | |
| 20 mM | 0.1936 mL | 0.9681 mL | 1.9363 mL | 4.8406 mL | |
| 25 mM | 0.1549 mL | 0.7745 mL | 1.5490 mL | 3.8725 mL | |
| 30 mM | 0.1291 mL | 0.6454 mL | 1.2908 mL | 3.2271 mL | |
| 40 mM | 0.0968 mL | 0.4841 mL | 0.9681 mL | 2.4203 mL | |
| 50 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
| 60 mM | 0.0645 mL | 0.3227 mL | 0.6454 mL | 1.6135 mL | |
| 80 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2102 mL | |
| 100 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9681 mL |
5-Hydroxymethyl-2'-deoxyuridine Related Classifications
HY-129983 Related Classifications
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