3′-Deoxythymidine
Based on 1 Customer Validation
3′-Deoxythymidine (2′,3′-Dideoxythymidine), a nucleoside analog, inhibits eukaryotic cellular and viral DNA polymerases, and inhibits retrovirus infection in some cells.
For research use only. We do not sell to patients.
- Purity: 99.13%
- CAS No.: 3416-05-5
- Formula: C10H14N2O4
- Molecular Weight:226.23
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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Biological Activity
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DNA Polymerase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| ATH-8 cell line | ED50 |
100 μM
Compound: 6a
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Protection of ATH8 cells against the cytopathic effect of HIV.
Protection of ATH8 cells against the cytopathic effect of HIV.
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[PMID: 3497272] |
| CCRF-CEM | IC50 |
0.38 μM
Compound: 5
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Concentration of compound required to inhibit 50% of viral replication of human immunodeficiency virus in CEM-F cells
Concentration of compound required to inhibit 50% of viral replication of human immunodeficiency virus in CEM-F cells
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[PMID: 2754712] |
| CEM-SS | CC50 |
>100 μM
Compound: ddT
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Cytotoxicity against human CEM-SS cells assessed as reduction in cell viability
Cytotoxicity against human CEM-SS cells assessed as reduction in cell viability
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[PMID: 34695774] |
| CEM-TK(-) | CC50 |
>100 μM
Compound: ddT
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Cytotoxicity against human CEM-TK(-) cells assessed as reduction in cell viability
Cytotoxicity against human CEM-TK(-) cells assessed as reduction in cell viability
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[PMID: 34695774] |
| MT2 | EC50 |
>200 μM
Compound: 4
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Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days
Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days
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[PMID: 17562366] |
| MT4 | CC50 |
>100 μM
Compound: ddT
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Cytotoxicity against human MT4 cells assessed as reduction in cell viability
Cytotoxicity against human MT4 cells assessed as reduction in cell viability
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[PMID: 34695774] |
| MT4 | ED50 |
6 μM
Compound: 4
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Concentration required to affect a 50% reduction in the cytopathic effect of HIV for MT-4 cells
Concentration required to affect a 50% reduction in the cytopathic effect of HIV for MT-4 cells
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[PMID: 1527788] |
Chemical Information
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CAS No. 3416-05-5
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Appearance Solid
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Molecular Weight 226.23
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Formula C10H14N2O4
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Color White to off-white
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SMILES
O=C(NC(C(C)=C1)=O)N1[C@@H]2O[C@H](CO)CC2
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Synonyms
2′,3′-Dideoxythymidine; ddThd
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (442.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (22.10 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (249 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.4203 mL | 22.1014 mL | 44.2028 mL | 110.5070 mL |
| 5 mM | 0.8841 mL | 4.4203 mL | 8.8406 mL | 22.1014 mL | |
| 10 mM | 0.4420 mL | 2.2101 mL | 4.4203 mL | 11.0507 mL | |
| 15 mM | 0.2947 mL | 1.4734 mL | 2.9469 mL | 7.3671 mL | |
| 20 mM | 0.2210 mL | 1.1051 mL | 2.2101 mL | 5.5254 mL | |
| 25 mM | 0.1768 mL | 0.8841 mL | 1.7681 mL | 4.4203 mL | |
| 30 mM | 0.1473 mL | 0.7367 mL | 1.4734 mL | 3.6836 mL | |
| 40 mM | 0.1105 mL | 0.5525 mL | 1.1051 mL | 2.7627 mL | |
| 50 mM | 0.0884 mL | 0.4420 mL | 0.8841 mL | 2.2101 mL | |
| 60 mM | 0.0737 mL | 0.3684 mL | 0.7367 mL | 1.8418 mL | |
| 80 mM | 0.0553 mL | 0.2763 mL | 0.5525 mL | 1.3813 mL | |
| 100 mM | 0.0442 mL | 0.2210 mL | 0.4420 mL | 1.1051 mL |
3′-Deoxythymidine Related Classifications
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