5-OH-HxMF 

5-OH-HxMF is a hydroxylated polymethoxyflavone that has anti-inflammatory, anticancer, neurotrophic and neuroprotective activities^[1][2][3].

5-OH-HxMF (5-20 µM; 48 h) effectively induces PC12 neurite outgrowth accompanied with the expression of neuronal differentiation marker protein growth-associated protein-43(GAP-43)^[1].
5-OH-HxMF (20 µM; 0-120 min) causes the enhancement of cyclic AMP response element binding protein (CREB) phosphorylation, c-fos gene expression and CRE-mediated transcription^[1].
5-OH-HxMF significantly reduces the production of nitric oxide and prostaglandin E2 and downregulates inducible nitric oxide synthase (iNOS) and COX-2 expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. 5-OH-HxMF inhibits the release of pro-inflammatory cytokines, such as tumor necrosis factor-α and IL-1β, and decreases the transcriptional levels. 5-OH-HxMF significantly inhibits the LPS-induced NF-κB translocation from the cytosol to the nucleus, which is associated with the abrogation of inhibitory IκBα degradation and subsequent decreases in nuclear p65 levels^[2].
5-OH-HxMF inhibits cell growth and induces apoptosis in human leukemia cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-OH-HxMF (topically treated; twice a week; for 20 weeks; 1 and 3 μmol in 200 μL acetone) is an effective antitumor agent and its inhibitory effect is through the down-regulation of inflammatory iNOS and COX-2 gene expression in mouse skin^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

418.39

C₂₁H₂₂O₉

1176-88-1

O=C1C(OC)=C(OC2=C1C(O)=C(C(OC)=C2OC)OC)C3=CC=C(C(OC)=C3)OC

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hui-Chi Lai, et al. Neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via cAMP/PKA/CREB pathway in PC12 cells. PLoS One. 2011;6(11):e28280.  [Content Brief]

  [2]. Min Jeong Kim, et al. Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone via NF-κB inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage. Mol Med Rep. 2014 Apr;9(4):1197-203.  [Content Brief]

  [3]. Ching-Shu Lai, et al. Inhibitory effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on 12-O-tetradecanoylphorbol 13-acetate-induced skin inflammation and tumor promotion in mice. Carcinogenesis. 2007 Dec;28(12):2581-8.  [Content Brief]