7ß,27-Dihydroxycholesterol
Based on 1 Customer Validation
7ß,27-Dihydroxycholesterol (7β, 27-OHC) is a potent and selective activator of RORγt (Ki=120 nM). 7ß,27-Dihydroxycholesterol promotes the differentiation of mouse and human CD4+ Th17 cells. 7ß,27-Dihydroxycholesterol also increases the production of IL-17 depended on CYP27A1.
For research use only. We do not sell to patients.
- Purity : 99.5%
- CAS No.: 240129-43-5
- Formula: C27H46O3
- Molecular Weight:418.65
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-113345
ZymostenolRORγ Agonist -
HY-14422
SR1078RORα/RORγ Agonist -
HY-125801
3-Oxo-5β-cholanoic acidTH17 Cell Differentiation Inhibitor -
HY-115408
9(R)-HETE -
HY-113943
9(S)-HETE9-HETE Isomer -
HY-101454
SR0987RORγt Agonist -
HY-104037
CintirorgonRORγ Agonist -
HY-120210
XY018ROR-γ Antagonist -
HY-111323
ASN06917370GPR17 ligand -
HY-113345R
Zymostenol (Standard)RORγ Agonist -
HY-49076
RORγ inverse agonist 1RORγ Inverse Agonist -
HY-131389
Cholesteryl heptadecanoateCholesteryl Ester Internal Standard
Biological Activity
Description
IC50 & Target
[1]|
RORγt 120 nM (Ki) |
In Vitro
In Vivo
Chemical Information
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CAS No. 240129-43-5
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Appearance Solid
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Molecular Weight 418.65
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Formula C27H46O3
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Color White to off-white
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SMILES
C[C@@H](CO)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])[C@@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Synonyms
7β, 27-OHC
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Initial Source
Human body
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
Ethanol : ≥ 20 mg/mL (47.77 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Naïve CD4+ T-cell subset differentiation/polarization
Naïve CD4+ T-cell subset differentiation/polarization is an in vitro assay in which purified naïve CD4+ T cells are activated through TCR and CD28 costimulation and cultured with defined cytokines and neutralizing antibodies to generate Th0, Th1, Th2, Th17, or induced Treg-like populations. Differentiation is detected by subset-associated cytokines and transcription factors: IFN-γ/T-bet for Th1, IL-4/GATA3 for Th2, IL-17A/RORγt for Th17, and Foxp3 for induced Treg cells. The assay readout is usually generated by intracellular cytokine staining after restimulation, transcription-factor staining by flow cytometry, ELISA of secreted cytokines, or gene-expression analysis. The result reflects cytokine-directed lineage commitment or polarization rather than antigen-specific immune protection by itself.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.3886 mL | 11.9432 mL | 23.8863 mL | 59.7158 mL |
| 5 mM | 0.4777 mL | 2.3886 mL | 4.7773 mL | 11.9432 mL | |
| 10 mM | 0.2389 mL | 1.1943 mL | 2.3886 mL | 5.9716 mL | |
| 15 mM | 0.1592 mL | 0.7962 mL | 1.5924 mL | 3.9811 mL | |
| 20 mM | 0.1194 mL | 0.5972 mL | 1.1943 mL | 2.9858 mL | |
| 25 mM | 0.0955 mL | 0.4777 mL | 0.9555 mL | 2.3886 mL | |
| 30 mM | 0.0796 mL | 0.3981 mL | 0.7962 mL | 1.9905 mL | |
| 40 mM | 0.0597 mL | 0.2986 mL | 0.5972 mL | 1.4929 mL |
7ß,27-Dihydroxycholesterol Related Classifications
HY-138115 Related Classifications
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