EPAC 5376753
Based on 1 Customer Validation
EPAC 5376753, a 2-Thiobarbituric acid (HY-77962) derivative, is a selective and allosteric Epac inhibitor. EPAC 5376753 inhibits Epac1 with an IC50 of 4 µM in Swiss 3T3 cells. EPAC 5376753 does not inhibit PKA and adenylyl cyclases.
For research use only. We do not sell to patients.
- Purity : 98.10%
- CAS No.: 302826-61-5
- Formula: C15H8Cl2N2O3S
- Molecular Weight:367.21
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
IC50: 4 µM (Epac)[1]
In Vitro
EPAC 5376753 (1-100 μM; 48 h) above 50 μM will significantly inhibit cell activity, and below 50 μM will have no effect on cell activity[1].
Epac is a key mediator of cAMP signaling. EPAC 5376753 selectively inhibits Epac-mediated migration in fibroblasts[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Swiss 3T3 cells
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Concentration:1 μM, 10 μM, 25 μM, 50 μM, 75 μM, 100 μM
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Incubation Time:48 h
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Result:<50 μM of compound did not significantly decrease viability.
>50 μM significantly inhibited cell activity.
Chemical Information
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CAS No. 302826-61-5
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Appearance Solid
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Molecular Weight 367.21
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Formula C15H8Cl2N2O3S
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Color Orange to red
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SMILES
O=C(/C(C(N1)=O)=C/C2=CC=C(C3=CC=C(Cl)C=C3Cl)O2)NC1=S
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
EPAC 5376753 Related Classifications
HY-111446 Related Classifications
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