2. GPCR/G Protein Neuronal Signaling
  3. Melanocortin Receptor
  4. Nonapeptide-1 acetate salt

Nonapeptide-1 acetate salt  (Synonyms: Melanostatine-5 acetate salt)

Cat. No.: HY-P0097A Purity: 98.70%
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Nonapeptide-1 (Melanostatine-5) acetate salt, a peptide hormone, is a selective antagonist of MC1R (Ki: 40 nM). Nonapeptide-1 acetate salt is a competitive α-MSH antagonist that potently inhibits intracellular cAMP and melanosome dispersion induced by α-MSH in melanocytes (IC50: 2.5 nM and 11 nM, respectively). Nonapeptide-1 acetate salt inhibits melanin synthesis, and can be used in the research of skin pigmentation and regulation of steroid production in the adrenal gland, skin cancer.

For research use only. We do not sell to patients.

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Nonapeptide-1 acetate salt Chemical Structure

Nonapeptide-1 acetate salt Chemical Structure

Size Price Stock Quantity
1 mg USD 80 In-stock
5 mg USD 220 In-stock
10 mg USD 320 In-stock
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100 mg   Get quote  

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Based on 2 publication(s) in Google Scholar

Other Forms of Nonapeptide-1 acetate salt:

Nonapeptide-1 acetate salt Related Antibodies

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Description

Nonapeptide-1 (Melanostatine-5) acetate salt, a peptide hormone, is a selective antagonist of MC1R (Ki: 40 nM). Nonapeptide-1 acetate salt is a competitive α-MSH antagonist that potently inhibits intracellular cAMP and melanosome dispersion induced by α-MSH in melanocytes (IC50: 2.5 nM and 11 nM, respectively). Nonapeptide-1 acetate salt inhibits melanin synthesis, and can be used in the research of skin pigmentation and regulation of steroid production in the adrenal gland, skin cancer[1][2][3].

IC50 & Target[2]

MC1R

 

MC3R

0.47 μM (Ki)

MC4R

1.34 μM (Ki)

MC5R

2.4 μM (Ki)

In Vitro

Nonapeptide-1 acetate salt (153N-6) inhibits α-melanocyte hormone (α-MSH)-induced melanosome dispersion, with an IC50 value of 11 nM[1].
Nonapeptide-1 acetate salt (0.1 nM-1 μΜ, 30 min) inhibits α-MSH-induced intracellular cAMP levels in melanocytes, with an IC50 of 2.5 nM[1].
Nonapeptide-1 acetate salt (153N-6) shows highest affinity for MC1R (Ki: 40 nM) in COS-1 cells expressing human receptors, and is selective for MC1R over MC3R, MC4R, and MC5R (Ki: 0.47, 1.34, and 2.4 μΜ, respectively)[2].
Nonapeptide-1 acetate salt (N-1A, 20 μΜ, 3 days) inhibits the basal melanin synthesis and reverses UVA-induced melanin increase in Human epidermal melanocytes (HEM cells) and HaCaT cells[3].
Nonapeptide-1 acetate salt (20 μΜ, 3 days) competes with α-MSH and downregulates the expression of MC1R, tyrosinase, TRP1, TRP2, and MITF via binding to MC1R in HaCaT cells and HEM cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nonapeptide-1 acetate salt Related Antibodies

Western Blot Analysis[3]

Cell Line: HaCaT cells, Human epidermal melanocytes (HEM)
Concentration: 20 μΜ
Incubation Time: 3 days
Result: Downregulated the expression of MC1R, tyrosinase, TRP1, TRP2, and MITF.
Molecular Weight

1266.56

Formula

C63H91N15O11S
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (39.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7895 mL 3.9477 mL 7.8954 mL
5 mM 0.1579 mL 0.7895 mL 1.5791 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (39.48 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.7895 mL 3.9477 mL 7.8954 mL 19.7385 mL
5 mM 0.1579 mL 0.7895 mL 1.5791 mL 3.9477 mL
10 mM 0.0790 mL 0.3948 mL 0.7895 mL 1.9739 mL
15 mM 0.0526 mL 0.2632 mL 0.5264 mL 1.3159 mL
20 mM 0.0395 mL 0.1974 mL 0.3948 mL 0.9869 mL
25 mM 0.0316 mL 0.1579 mL 0.3158 mL 0.7895 mL
30 mM 0.0263 mL 0.1316 mL 0.2632 mL 0.6580 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Nonapeptide-1 acetate salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nonapeptide-1Melanostatine-5Nonapeptide1Nonapeptide 1Melanostatine5Melanostatine 5Melanocortin ReceptorMC ReceptorSkin pigmentationmelaninmelanogenesisUVAHaCaTHEMmelanocytesInhibitorinhibitorinhibit

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