Pim1/AAK1-IN-1
Based on 13 publication(s) in Google Scholar
Pim1/AAK1-IN-1 is a potent multi-kinase inhibitor with Kd values of 35 nM/53 nM/75 nM/380 nM for Pim1/AAK1/MST2/LKB1 respectively, and also inhibits MPSK1 and TNIK.
For research use only. We do not sell to patients.
- Purity: 98.40%
- CAS No.: 1093222-27-5
- Formula: C20H13N5O
- Molecular Weight:339.35
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Epigenetics Compound Library
HY-L005
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Immunology/Inflammation Compound Library
HY-L007
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JAK/STAT Compound Library
HY-L008
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Kinase Inhibitor Library
HY-L009
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Anti-Cancer Compound Library
HY-L025
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Anti-Pancreatic Cancer Compound Library
HY-L077
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Anti-Cancer Metabolism Compound Library
HY-L083
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Anti-Obesity Compound Library
HY-L087
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Cancer Stem Cells Compound Library
HY-L135
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Heterocyclic Compound Library
HY-L138
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MCE Bioactive Compound Library
HY-L001V
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Protein Kinase Compound Library
HY-L196
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) Pim1/AAK1-IN-1
More- Nat Metab. 2022 Sep;4(9):1185-1201. [Abstract]
- J Adv Res. 2024 Dec 2:S2090-1232(24)00555-1. [Abstract]
- Microbiome. 2025 Feb 4;13(1):36. [Abstract]
- Phytomedicine. 2025 Apr:139:156563. [Abstract]
- Cell Chem Biol. 2026 Jun 18;33(6):767-784.e7. [Abstract]
- EMBO J. 2025 Sep 9. [Abstract]
- EMBO J. 2021 Nov 2;40(21):e108028. [Abstract]
- Cell Rep. 2022 Aug 16;40(7):111188. [Abstract]
- Life Sci. 2025 Jun 1:370:123564. [Abstract]
- Commun Biol. 2025 Nov 23. [Abstract]
- FASEB J. 2026 Mar 31;40(6):e71712. [Abstract]
- Mol Biol Rep. 2024 Apr 21;51(1):562. [Abstract]
- Animals (Basel). 2022 Sep 19;12(18):2474. [Abstract]
Biological Activity
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PIM1 |
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Cell Line
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Type | Value | Description | References |
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| Huh-7 | CC50 |
>10 μM
Compound: 1
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Cytotoxicity against human HuH7 cells infected with Ebolavirus Kikwit isolate after 48 hrs by Celltiter-glo reagent based luminescence assay
Cytotoxicity against human HuH7 cells infected with Ebolavirus Kikwit isolate after 48 hrs by Celltiter-glo reagent based luminescence assay
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[PMID: 31136173] |
| Huh-7 | CC50 |
>50 μM
Compound: 1
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Cytotoxicity against human HuH7 cells infected with DENV2 New Guinea C by microplate alamar blue assay
Cytotoxicity against human HuH7 cells infected with DENV2 New Guinea C by microplate alamar blue assay
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[PMID: 31136173] |
| Huh-7 | CC50 |
>50 μM
Compound: 7
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Cytotoxicity against human Huh-7 cells
Cytotoxicity against human Huh-7 cells
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[PMID: 38377825] |
| Vero | EC50 |
>10 μM
Compound: 1
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Antiviral activity against Ebolavirus Kikwit isolate infected in African green monkey Vero cells assessed as plaque reduction after 48 hrs by neutral red-staining based assay
Antiviral activity against Ebolavirus Kikwit isolate infected in African green monkey Vero cells assessed as plaque reduction after 48 hrs by neutral red-staining based assay
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[PMID: 31136173] |
| Vero C1008 | CC50 |
>50 μM
Compound: 109
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Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 96 hrs by MTS assay
Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 96 hrs by MTS assay
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[PMID: 37981443] |
Chemical Information
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CAS No. 1093222-27-5
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Appearance Solid
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Molecular Weight 339.35
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Formula C20H13N5O
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Color White to gray
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SMILES
N#CC1=CC=C(C2=CN=C3C(C(NC(C4=CC=CN=C4)=O)=CN3)=C2)C=C1
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Synonyms
LKB1/AAK1 dual inhibitor
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
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Journal Impact Factor
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Most Recent
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Nat Metab
Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis. [Abstract]2022 Sep;4(9):1185-1201. PMID: 36050503 -
J Adv Res
Targeting Dlat-Trpv3 pathway by hyperforin elicits non-canonical promotion of adipose thermogenesis as an effective anti-obesity strategy. [Abstract]2024 Dec 2:S2090-1232(24)00555-1. PMID: 39631519 -
Microbiome
Microbiota governs host chenodeoxycholic acid glucuronidation to ameliorate bile acid disorder induced diarrhea. [Abstract]2025 Feb 4;13(1):36. PMID: 39905483 -
Phytomedicine
2025 Apr:139:156563. PMID: 40023068 -
Cell Chem Biol
The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]2026 Jun 18;33(6):767-784.e7. PMID: 42061410 -
EMBO J
Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development. [Abstract]2025 Sep 9. PMID: 40926123 -
EMBO J
2021 Nov 2;40(21):e108028. PMID: 34472622 -
Cell Rep
Neuronal paxillin and drebrin mediate BDNF-induced force transduction and growth cone turning in a soft-tissue-like environment. [Abstract]2022 Aug 16;40(7):111188. PMID: 35977504 -
Life Sci
LKB1 activated by NaB inhibits the IL-4/STAT6 axis and ameliorates renal fibrosis through the suppression of M2 macrophage polarization. [Abstract]2025 Jun 1:370:123564. PMID: 40097066 -
Commun Biol
Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis. [Abstract]2025 Nov 23. PMID: 41276610 -
FASEB J
Regulator of Calcineurin 1 Aggravates Tubular Epithelial Cell Injury in Diabetic Mice Through Promoting Liver Kinase B1 Ubiquitination. [Abstract]2026 Mar 31;40(6):e71712. PMID: 41854627 -
Mol Biol Rep
Dapagliflozin promotes browning of white adipose tissue through the FGFR1-LKB1-AMPK signaling pathway. [Abstract]2024 Apr 21;51(1):562. PMID: 38644407 -
Animals (Basel)
Molecular Characterization of LKB1 of Triploid Crucian Carp and Its Regulation on Muscle Growth and Quality. [Abstract]2022 Sep 19;12(18):2474. PMID: 36139343
Solvent & Solubility
DMSO : 50 mg/mL (147.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9468 mL | 14.7341 mL | 29.4681 mL | 73.6703 mL |
| 5 mM | 0.5894 mL | 2.9468 mL | 5.8936 mL | 14.7341 mL | |
| 10 mM | 0.2947 mL | 1.4734 mL | 2.9468 mL | 7.3670 mL | |
| 15 mM | 0.1965 mL | 0.9823 mL | 1.9645 mL | 4.9114 mL | |
| 20 mM | 0.1473 mL | 0.7367 mL | 1.4734 mL | 3.6835 mL | |
| 25 mM | 0.1179 mL | 0.5894 mL | 1.1787 mL | 2.9468 mL | |
| 30 mM | 0.0982 mL | 0.4911 mL | 0.9823 mL | 2.4557 mL | |
| 40 mM | 0.0737 mL | 0.3684 mL | 0.7367 mL | 1.8418 mL | |
| 50 mM | 0.0589 mL | 0.2947 mL | 0.5894 mL | 1.4734 mL | |
| 60 mM | 0.0491 mL | 0.2456 mL | 0.4911 mL | 1.2278 mL | |
| 80 mM | 0.0368 mL | 0.1842 mL | 0.3684 mL | 0.9209 mL | |
| 100 mM | 0.0295 mL | 0.1473 mL | 0.2947 mL | 0.7367 mL |
Pim1/AAK1-IN-1 Related Classifications
HY-10371 Related Classifications
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