QL9

Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with K^b- or L^d-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×10⁴) are incubated with T2-K^b (7.5×10⁴) or T2-L^d (7.5×10⁴) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO₂. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A_{450 (sample)}-A_{450(no peptide)})/(Max A_450(sample)-A_{450(no peptide)}))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD₅₀) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.