Selegiline hydrochloride
Based on 11 publication(s) in Google Scholar
Selegiline (Deprenyl) hydrochloride is a potent, selective and irreversible inhibitor of MAO-B, with an IC50 of 51 nM. Selegiline hydrochloride exhibits 450-flod selectivity for MAO-B over MAO-A (IC50=23 μM). Selegiline hydrochloride can be used for the research of Parkinson's disease, Alzheimer's disease and major depressive disorder.
For research use only. We do not sell to patients.
- Purity : 99.94%
- CAS No.: 14611-52-0
- Formula: C13H18ClN
- Molecular Weight:223.74
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Selegiline hydrochloride
More- Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
- J Neuroinflammation. 2023 Jul 11;20(1):162. [Abstract]
- Proc Natl Acad Sci U S A. 2020 Oct 20;117(42):26438-26447. [Abstract]
- Anal Chem. 2025 Jul 15;97(27):14220-14229. [Abstract]
- Nutrients. 2023 May 30;15(11):2542. [Abstract]
- Eur J Pharm Sci. 2026 Jul 8:107603.
- Tissue Cell. 2026 Apr:99:103257. [Abstract]
- Head Neck. 2024 Aug;46(8):2031-2041. [Abstract]
- Cell Biomater. 2025 Dec 18.
- Research Square Preprint. 2024 Oct 09.
- bioRxiv. 2020 Feb.
Customer Validation & Images
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In Vivo Efficacy Study
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WB
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IHC
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ELISA
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WB
Biological Activity
Description
IC50 & Target
[1]|
MAO-B 51 nM (IC50) |
In Vitro
Selegiline (1 nM-1 μM) hydrochloride inhibits recombinant human MAO-B in a concentration-dependent manner[1].
Selegiline (10 mM) hydrochloride increases the efflux of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) from the rat hypothalamus in vitro. Selegiline hydrochloride inhibits the efflux of dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) in a dose-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6J mice (20-22 g)[4]
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Dosage:1 mg/kg
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Administration:I.p. daily for 24 days
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Result:Single injection did not acutely affect mouse locomotor activity.
14 consecutive days administration did not affect food reward.
Reduced Cocaine self-administration, and reduced DOPAC and 5-HIAA concentrations in frontal cortex.
Chemical Information
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CAS No. 14611-52-0
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Appearance Solid
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Molecular Weight 223.74
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Formula C13H18ClN
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Color White to off-white
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SMILES
CN(CC#C)[C@H](C)CC1=CC=CC=C1.Cl
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Synonyms
Deprenyl hydrochloride; (-)-Selegiline hydrochloride; (-)-Deprenyl hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (11)
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Journal Impact Factor
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Most Recent
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Cell Commun Signal
Fasting-mimicking diet alleviates inflammatory pain by inhibiting neutrophil extracellular traps formation and neuroinflammation in the spinal cord. [Abstract]2023 Sep 21;21(1):250. PMID: 37735678
Selegiline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
Schematic of the experimental timeline for analyzing the effects of the MAO-B inhibitor Selegiline hydrochloride (10 mg/kg, ip, 7 days) on CFA-induced allodynia and NETs formation.
Selegiline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
Representative blots and quantification of MPO and citH3 in the spinal cords of vehicle, Selegiline hydrochloride, selegiline + CI-amidine, and Selegiline hydrochloride (10 mg/kg, ip, 7 days) + PMA groups.
Selegiline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
Double immunofluorescence staining for citH3 (red) and MPO (green) in the spinal cords. NETs visualized by colocalization of citH3 and MPO staining (merged images; n = 3, scale bars: 50 or 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the vehicle group mice. #P < 0.05, compared with the Selegiline hydrochloride (10 mg/kg, ip, 7 days) group mice.
Selegiline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
ELISAs detecting IL-1β, IL-6, and TNF-α in the spinal cords (n = 5, IL-1β: F3, 16 = 35.98, P < 0.0001; IL-6: F3, 16 = 58.79, P < 0.0001; TNF-α: F3, 16 = 155.5, P < 0.0001). The expression levels in the vehicle group were set to 1 for quantification. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the vehicle group mice. #P < 0.05, compared with the Selegiline hydrochloride (10 mg/kg, ip, 7 days) group mice.
Selegiline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 Sep 21;21(1):250. [Abstract]
Representative blots and quantification of GPR35 in the spinal cords of vehicle, FMD, FMD + Selegiline hydrochloride (10 mg/kg, ip, 7 days), and FMD + 5-HIAA + PMA groups (n = 5, F3, 16 = 23.56, P < 0.0001).
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J Neuroinflammation
NLRP3-GABA signaling pathway contributes to the pathogenesis of impulsive-like behaviors and cognitive deficits in aged mice. [Abstract]2023 Jul 11;20(1):162. PMID: 37434240 -
Proc Natl Acad Sci U S A
2020 Oct 20;117(42):26438-26447. PMID: 33024014 -
Anal Chem
2025 Jul 15;97(27):14220-14229. PMID: 40601392 -
Nutrients
Inhibition of MAOB Ameliorated High-Fat-Diet-Induced Atherosclerosis by Inhibiting Endothelial Dysfunction and Modulating Gut Microbiota. [Abstract]2023 May 30;15(11):2542. PMID: 37299505 -
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Tissue Cell
POU6F1 inhibits cervical cancer progression by activating MAOB to block the ERK1/2 pathway. [Abstract]2026 Apr:99:103257. PMID: 41371117 -
Head Neck
Monoamine oxidase B inhibits epithelial-mesenchymal transition and trigger apoptosis via targeting ERK1/2 signaling pathway in head and neck squamous cell carcinoma. [Abstract]2024 Aug;46(8):2031-2041. PMID: 38379404 -
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Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (446.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 33.33 mg/mL (148.97 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (22.35 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (22.35 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Chaurasiya ND, et, al. Selective Inhibition of Human Monoamine Oxidase B by Acacetin 7-Methyl Ether Isolated from Turnera diffusa (Damiana). Molecules. 2019 Feb 23;24(4):810. [Content Brief]
[2]. Tatton WG, et, al. Modulation of gene expression rather than monoamine oxidase inhibition: (-)-deprenyl-related compounds in controlling neurodegeneration. Neurology. 1996 Dec;47(6 Suppl 3):S171-83. [Content Brief]
[3]. MohanKumar PS, et, al. Deprenyl stimulates the efflux of monoamines from the rat hypothalamus in vitro. Brain Res Bull. 2001 Apr;54(6):675-80. [Content Brief]
[4]. Ho MC, et, al. Chronic treatment with monoamine oxidase-B inhibitors decreases cocaine reward in mice. Psychopharmacology (Berl). 2009 Jul;205(1):141-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 4.4695 mL | 22.3474 mL | 44.6947 mL | 111.7368 mL |
| 5 mM | 0.8939 mL | 4.4695 mL | 8.9389 mL | 22.3474 mL | |
| 10 mM | 0.4469 mL | 2.2347 mL | 4.4695 mL | 11.1737 mL | |
| 15 mM | 0.2980 mL | 1.4898 mL | 2.9796 mL | 7.4491 mL | |
| 20 mM | 0.2235 mL | 1.1174 mL | 2.2347 mL | 5.5868 mL | |
| 25 mM | 0.1788 mL | 0.8939 mL | 1.7878 mL | 4.4695 mL | |
| 30 mM | 0.1490 mL | 0.7449 mL | 1.4898 mL | 3.7246 mL | |
| 40 mM | 0.1117 mL | 0.5587 mL | 1.1174 mL | 2.7934 mL | |
| 50 mM | 0.0894 mL | 0.4469 mL | 0.8939 mL | 2.2347 mL | |
| 60 mM | 0.0745 mL | 0.3725 mL | 0.7449 mL | 1.8623 mL | |
| 80 mM | 0.0559 mL | 0.2793 mL | 0.5587 mL | 1.3967 mL | |
| 100 mM | 0.0447 mL | 0.2235 mL | 0.4469 mL | 1.1174 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Selegiline hydrochloride Related Classifications
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