2. Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Endogenous Metabolite Apoptosis HBV Bacterial
  4. Thiamine hydrochloride

Thiamine hydrochloride  (Synonyms: Thiamine chloride hydrochloride; Vitamin B1 hydrochloride)

Cat. No.: HY-N0680 Purity: 99.99%
COA Handling Instructions

Molarity Calculator

Thiamine hydrochloride (Thiamine chloride hydrochloride) is an essential micronutrient needed as a cofactor for many central metabolic enzymes.

For research use only. We do not sell to patients.

Thiamine hydrochloride Chemical Structure

Thiamine hydrochloride Chemical Structure

CAS No. : 67-03-8

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
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1 g USD 84 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine hydrochloride:

Thiamine hydrochloride Related Antibodies

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Description

Thiamine hydrochloride (Thiamine chloride hydrochloride) is an essential micronutrient needed as a cofactor for many central metabolic enzymes.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

Molecular Weight

337.27

Formula

C12H18Cl2N4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(CCO)SC=[N+]1CC2=CN=C(C)N=C2N.[Cl-].Cl
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (296.50 mM; Need ultrasonic)

DMSO : 6.67 mg/mL (19.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9650 mL 14.8249 mL 29.6498 mL
5 mM 0.5930 mL 2.9650 mL 5.9300 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (3.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.67 mg/mL (1.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (98.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.99%

COA (249 KB) HNMR (119 KB) RP-HPLC (181 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.9650 mL 14.8249 mL 29.6498 mL 74.1246 mL
5 mM 0.5930 mL 2.9650 mL 5.9300 mL 14.8249 mL
10 mM 0.2965 mL 1.4825 mL 2.9650 mL 7.4125 mL
15 mM 0.1977 mL 0.9883 mL 1.9767 mL 4.9416 mL
H2O 20 mM 0.1482 mL 0.7412 mL 1.4825 mL 3.7062 mL
25 mM 0.1186 mL 0.5930 mL 1.1860 mL 2.9650 mL
30 mM 0.0988 mL 0.4942 mL 0.9883 mL 2.4708 mL
40 mM 0.0741 mL 0.3706 mL 0.7412 mL 1.8531 mL
50 mM 0.0593 mL 0.2965 mL 0.5930 mL 1.4825 mL
60 mM 0.0494 mL 0.2471 mL 0.4942 mL 1.2354 mL
80 mM 0.0371 mL 0.1853 mL 0.3706 mL 0.9266 mL
100 mM 0.0296 mL 0.1482 mL 0.2965 mL 0.7412 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Thiamine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiamine67-03-8Thiamine chlorideVitamin B1Vitamin B 1Vitamin B-1Endogenous MetaboliteApoptosisHBVBacterialHepatitis B virusInhibitorinhibitorinhibit

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