Cat. No.: HY-N0682

Pyridoxine hydrochloride

(Synonyms: Vitamin B6 hydrochloride)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Pyridoxine hydrochloride (Pyridoxol; Vitamin B6) is a pyridine derivative. Pyridoxine (Pyridoxol; Vitamin B6) exerts antioxidant effects in cell model of Alzheimer's disease via the Nrf-2/HO-1 pathway.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 58-56-0
Formula: C₈H₁₂ClNO₃
Molecular Weight:205.64

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Pyridoxine hydrochloride Chemical Structure

CAS No.: 58-56-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Pyridoxine exerts a protective potential against AD, attenuates ROS levels, decreases the expressions of cytoplasmic Nrf2, and upregulates whole-cell HO-1 expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 58-56-0
Appearance Solid
Molecular Weight 205.64
Formula C₈H₁₂ClNO₃
Color White to off-white
SMILES

OCC1=C(CO)C(O)=C(C)N=C1.Cl
Synonyms

Vitamin B6 hydrochloride
Structure Classification
- Alkaloids
- Pyridine Alkaloids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (7)

All IFWBBio/Physico-chemical AssayRT-PCR

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Nat Commun

Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. [Abstract]

2020 Feb 18;11(1):941. PMID: 32071304

Pyridoxine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Pyridoxine (1-80 μM; 12 h) had a greater effect on increasing GSH synthesis with lower cytotoxicity than vindoline in primary astrocytes.

Pyridoxine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Pyridoxine (5 μM; 12 h) resulted in PKM2 dimerization in astrocytes.

Pyridoxine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Pyridoxine (5 μM; 12 h) increased PKM2 binding to Nrf2 in astrocytes.

Pyridoxine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Pyridoxine (5 μM; 12 h) enhanced Nrf2 binding to the promoters of Gclc/Gclm in astrocytes.

Pyridoxine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Pyridoxine (5 μM; 12 h) promoted the mRNA expression of Gclc and Gclm in astrocytes.
Microbiome

Species-specific enhancement of enterohemorrhagic E. coli pathogenesis mediated by microbiome metabolites. [Abstract]

2019 Mar 20;7(1):43. PMID: 30890187
Cancer Lett

Pyridoxine induces monocyte-macrophages death as specific treatment of acute myeloid leukemia. [Abstract]

2020 Nov 1:492:96-105. PMID: 32860849
Dev Cell

Vitamin B6 preserves the stemness-like phenotypes and antitumor ability of CD8+ T cells. [Abstract]

2025 Nov 27:S1534-5807(25)00691-4. PMID: 41314217
Molecules

Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]

2023 Apr 11;28(8):3375. PMID: 37110608
Laurea Magistrale in Biomedical Engineering, Politecnico di Milano

2019 Jun.

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (243.14 mM)

DMSO : ≥ 50 mg/mL (243.14 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8629 mL

24.3143 mL

48.6287 mL

5 mM

0.9726 mL

4.8629 mL

9.7257 mL

10 mM

0.4863 mL

2.4314 mL

4.8629 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (486.29 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li C, et al. Pyridoxine exerts antioxidant effects in cell model of Alzheimer's disease via the Nrf-2/HO-1 pathway. Cell Mol Biol (Noisy-le-grand). 2018 Jul 30;64(10):119-124. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.8629 mL	24.3143 mL	48.6287 mL	121.5717 mL
	5 mM	0.9726 mL	4.8629 mL	9.7257 mL	24.3143 mL
	10 mM	0.4863 mL	2.4314 mL	4.8629 mL	12.1572 mL
	15 mM	0.3242 mL	1.6210 mL	3.2419 mL	8.1048 mL
	20 mM	0.2431 mL	1.2157 mL	2.4314 mL	6.0786 mL
	25 mM	0.1945 mL	0.9726 mL	1.9451 mL	4.8629 mL
	30 mM	0.1621 mL	0.8105 mL	1.6210 mL	4.0524 mL
	40 mM	0.1216 mL	0.6079 mL	1.2157 mL	3.0393 mL
	50 mM	0.0973 mL	0.4863 mL	0.9726 mL	2.4314 mL
	60 mM	0.0810 mL	0.4052 mL	0.8105 mL	2.0262 mL
	80 mM	0.0608 mL	0.3039 mL	0.6079 mL	1.5196 mL
	100 mM	0.0486 mL	0.2431 mL	0.4863 mL	1.2157 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Pyridoxine hydrochloride

7 Cited Publications

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Pyridoxine hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Pyridoxine hydrochloride

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pyridoxine hydrochloride Related Classifications

HY-N0682 Related Classifications