Choline chloride

Name: Choline chloride
Brand: MedChemExpress
SKU: HY-B1337
Rating: 5.0 (4 reviews)

Cat. No.: HY-B1337 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Choline chloride is an essential nutrient that activates alpha7 nicotinic receptors and has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders.

For research use only. We do not sell to patients.

CAS No. : 67-48-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Choline chloride:

4 Publications Citing Use of MCE Choline chloride

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

RT-PCR

In Vivo Efficacy Study

: Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573. [Abstract]; Choline (30 μM, 24 h) demonstrated no cytotoxic effects but partially mitigated the inhibition of cell viability induced by EHDPP in HTR-8/SVneo and JEG-3 cells.

: Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573. [Abstract]; Choline (30 μM, 24 h) reversed EHDPP-induced cell migration inhibition in HTR-8/SVneo cells.

: Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573. [Abstract]; Choline (30 μM, 24 h) reversed the EHDPP-induced decrease of mRNA levels of TNF-α, IL-6, and IL-1β in HTR-8/SVneo cells.

: Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573. [Abstract]; Choline supplementation (25 mM choline-containing water for 31.5 days) significantly increased uterine size, fetal weight, placental weight, and placental diameter that were reduced by EHDPP exposure.

: Choline chloride purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Jun 5:489:137573. [Abstract]; Choline supplementation (25 mM choline-containing water for 31.5 days) alleviated the decrease in mRNA expression of the proliferation marker Ki67 and the vascular differentiation marker CD31 induced by EHDPP.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	17 μM Compound: 6209	Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells (PubChem AID: 1794811) Substrate uptake and inhibition of the High Affinity Choline Transporter 1 (CHT1, SLC5A7) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC5A7 cells (PubChem AID: 1794811)	10.5281/zenodo.7361355

In Vitro

Choline chloride (0 or 70 μM, 4 days) can effectively mitigate apoptosis and maintain cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Rat pheochromocytoma cells PC12
Concentration:	0 or 70 μM
Incubation Time:	4 days
Result:	Showed the cell viability of 94% at 70 μM while cell viability of 83% at 0 μM. Reduced the number of cells with DNA breaks (characteristic of apoptosis) by 8.5% at 70 μM compared to the no treatment group.

In Vivo

Choline chloride (s.c., 0.2 and 100 mg/kg/h, 24 or 48 hours) can reduce postoperative injurious reflexes and effectively decreases tumor necrosis factor (TNF) release from macrophages in female C57/Bl6 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Postoperative pain model of female C57/Bl6 mice^[2]
Dosage:	0.2 and 100 mg/kg/h
Administration:	Subcutaneous injection, 24 or 48 hours
Result:	Reduced heat hypersensitivity after surgery with maximal efficacy after 48 h treatment and the ED₅₀ value of choline dose was 1.7 mg/kg/h. Reduced hypersensitivity to punctate mechanical stimuli 48 hours after infusion in a dose-dependent manner and with the ED₅₀ value of 4.7 mg/kg/h but not 24 hours.

Clinical Trial

Molecular Weight

139.62

Formula

C₅H₁₄ClNO

CAS No.

67-48-1

Appearance

Solid

Color

White to off-white

SMILES

OCC[N+](C)(C)C.[Cl-]

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : ≥ 140 mg/mL (1002.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (716.23 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.1623 mL	35.8115 mL	71.6230 mL
5 mM	1.4325 mL	7.1623 mL	14.3246 mL
10 mM	0.7162 mL	3.5811 mL	7.1623 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 130 mg/mL (931.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (275 KB) SDS (396 KB)

COA (245 KB) HNMR (116 KB) RP-HPLC (242 KB)

Handling Instructions (2659 KB)

References

[1]. M Q Holmes-McNary, et al. Apoptosis is induced by choline deficiency in fetal brain and in PC12 cells. Brain Res Dev Brain Res. 1997 Jul 18;101(1-2):9-16. [Content Brief]

[2]. T J Rowley, et al. Antinociceptive and anti-inflammatory effects of choline in a mouse model of postoperative pain. Br J Anaesth. 2010 Aug;105(2):201-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	7.1623 mL	35.8115 mL	71.6230 mL	179.0574 mL
	5 mM	1.4325 mL	7.1623 mL	14.3246 mL	35.8115 mL
	10 mM	0.7162 mL	3.5811 mL	7.1623 mL	17.9057 mL
	15 mM	0.4775 mL	2.3874 mL	4.7749 mL	11.9372 mL
	20 mM	0.3581 mL	1.7906 mL	3.5811 mL	8.9529 mL
	25 mM	0.2865 mL	1.4325 mL	2.8649 mL	7.1623 mL
	30 mM	0.2387 mL	1.1937 mL	2.3874 mL	5.9686 mL
	40 mM	0.1791 mL	0.8953 mL	1.7906 mL	4.4764 mL
	50 mM	0.1432 mL	0.7162 mL	1.4325 mL	3.5811 mL
	60 mM	0.1194 mL	0.5969 mL	1.1937 mL	2.9843 mL
	80 mM	0.0895 mL	0.4476 mL	0.8953 mL	2.2382 mL
	100 mM	0.0716 mL	0.3581 mL	0.7162 mL	1.7906 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.