1. Neuronal Signaling
  2. AChE
  3. Hemicholinium 3

Hemicholinium 3 (Synonyms: Hemicholinium dibromide)

Cat. No.: HY-B2152
Handling Instructions

Hemicholinium 3 is a competitive inhibitor of the high affinity choline transporter (HACU) with a Ki value of 25 nM. Hemicholinium 3, a neuromuscular blocking agent which inhibits the synthesis and the release of acetylcholine (ACh). Hemicholinium 3 inhibits the Epibatidine-evoked contraction and [3H]acetylcholine release with IC50s of 897 nM and 693 nM, respectively.

For research use only. We do not sell to patients.

Hemicholinium 3 Chemical Structure

Hemicholinium 3 Chemical Structure

CAS No. : 312-45-8

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Description

Hemicholinium 3 is a competitive inhibitor of the high affinity choline transporter (HACU) with a Ki value of 25 nM. Hemicholinium 3, a neuromuscular blocking agent which inhibits the synthesis and the release of acetylcholine (ACh)[1]. Hemicholinium 3 inhibits the Epibatidine-evoked contraction and [3H]acetylcholine release with IC50s of 897 nM and 693 nM, respectively[2].

In Vitro

Hemicholinium 3 (HC-3) inhibits the presynaptic nicotinic acetylcholine receptors of myenteric neurons. HC-3 inhibits nAChRs located on the terminal region of myenteric neurons of guinea-pig longitudinal muscle strip preparation[2].
Hemicholinium 3 (HC-3) inhibits[3H]choline uptake with the Ki value of 13.3 μM in NCI-H69 cells[3].
HC-3 (1 mM) significantly inhibits cell viability and increases caspase-3/7 activity in NCI-H69 cells[3].
Hemicholinium 3 (Hemicholinium-3) inhibits sodium dependent high affinity choline uptake (IC50=18 nM)[4]. In vivo:

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: The human small cell lung carcinoma cell line NCI-H69
Concentration: 1 mM
Incubation Time: Added every day for 2 days.
Result: Markedly inhibited cell viability.
In Vivo

Hemicholinium-3 impairs spatial learning. HC-3 (2.5, 5.0 μg/rat/ICV; 1 h before training) dose dependently impairs spatial learning[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

574.35

Formula

C₂₄H₃₄Br₂N₂O₄

CAS No.
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