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  3. Thiamine nitrate

Thiamine nitrate  (Synonyms: Vitamin B1 nitrate)

Cat. No.: HY-B2223 Purity: 99.41%
COA Handling Instructions

Thiamine nitrate is an essential vitamin which can enhance normal neuronal actives.

For research use only. We do not sell to patients.

Thiamine nitrate Chemical Structure

Thiamine nitrate Chemical Structure

CAS No. : 532-43-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 28 In-stock
Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
500 mg USD 25 In-stock
1 g USD 30 In-stock
5 g USD 50 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine nitrate:

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Thiamine nitrate is an essential vitamin which can enhance normal neuronal actives.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Thiamine levels in the blood of homozygous KO and KI mice fed a conventional diet are decreased to 0.058±0.051 and 0.126±0.092 μM, respectively, at 7 weeks compare to WT mice (0.796±0.259 μM). When WT and homozygous KO and KI mice are fed a Thiamine-restricted diet (Thiamine: 0.60 mg/100 g food), blood Thiamine concentration at 5 and 14 days is markedly decreased to 0.010±0.009 and 0.010±0.006 μM, respectively, compare to WT mice (0.609±0.288 μM). Thiamine concentration in brain homogenate of WT mice fed a conventional diet is 3.81±2.18 nmol/g wet weight, and that of KO and KI is 1.33±0.96 and 2.16±1.55 nmol/g wet weight, respectively. Notably, Thiamine concentration in brain homogenate decreases steadily in KO and KI mice fed a thiamine-restrict diet (Thiamine: 0.60 mg/100 g food) for 5 days (0.95±0.72 nmol/g wet weight) and 14 days (1.11±0.24 nmol/g wet weight), respectively, compare to WT (3.65±1.02 nmol/g wet weight), before the mice presenting an phenotype of disease[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

WT, homozygous, and heterozygous KO and KI mice fed a conventional diet (thiamine: 1.71 mg/100 g) survive for over 6 months without any phenotype of disease. Homozygous KO and KI mice fed a Thiamine-restricted diet (thiamine: 0.60 mg/100 g food) show paralysis, weight loss, and immobility, and die within 12 and 30 days, respectively. Similarly, homozygous KO and KI mice fed a Thiamine-restricted diet with an even lower percentage of Thiamine (Thiamine: 0.27 mg/100 g food) die within 14 and 18 days, respectively. However, WT and heterozygous KO and KI mice fed a Thiamine-restricted diet (Thiamine: 0.60 mg or 0.27 mg/100g food) survive for over 6 months without any phenotype of disease[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

327.36

Formula

C12H17N5O4S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(CCO)SC=[N+]1CC2=CN=C(C)N=C2N.[O-][N+]([O-])=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (61.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0547 mL 15.2737 mL 30.5474 mL
5 mM 0.6109 mL 3.0547 mL 6.1095 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Animal Administration
[2]

Slc19a3 E314Q KI mice are maintained routinely with conventional diet, which has a Thiamine concentration (thiamine hydrochloride, MW=337.3) of 1.71 mg/100 g food. Two types of Thiamine-restrict food base on “purified diets for laboratory rodents” are prepared, in which Thiamine concentration is 0.60 mg/100 g food (35% Thiamine of conventional food) or 0.27 mg/100 g food (16% Thiamine of conventional food). A high-Thiamine-containing food is also prepared from AIN-93M, in which Thiamine concentration is five times that of CE-2 (thiamine: 8.50 mg/100 g food)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0547 mL 15.2737 mL 30.5474 mL 76.3685 mL
5 mM 0.6109 mL 3.0547 mL 6.1095 mL 15.2737 mL
10 mM 0.3055 mL 1.5274 mL 3.0547 mL 7.6369 mL
15 mM 0.2036 mL 1.0182 mL 2.0365 mL 5.0912 mL
20 mM 0.1527 mL 0.7637 mL 1.5274 mL 3.8184 mL
25 mM 0.1222 mL 0.6109 mL 1.2219 mL 3.0547 mL
30 mM 0.1018 mL 0.5091 mL 1.0182 mL 2.5456 mL
40 mM 0.0764 mL 0.3818 mL 0.7637 mL 1.9092 mL
50 mM 0.0611 mL 0.3055 mL 0.6109 mL 1.5274 mL
60 mM 0.0509 mL 0.2546 mL 0.5091 mL 1.2728 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Thiamine nitrate
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