1. Neuronal Signaling
  2. Amyloid-β
  3. β-Amyloid 22-35

β-Amyloid 22-35 (Synonyms: Amyloid β-Protein (22-35))

Cat. No.: HY-P1474
Handling Instructions

β-Amyloid (22-35) is a 14-aa peptide, shows aggregates and induces neurotoxicity in the hippocampal cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

β-Amyloid 22-35 Chemical Structure

β-Amyloid 22-35 Chemical Structure

CAS No. : 144189-71-9

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Description

β-Amyloid (22-35) is a 14-aa peptide, shows aggregates and induces neurotoxicity in the hippocampal cells.

IC50 & Target

Amyloid-β[1]

In Vitro

β-Amyloid (22-35) shows significant cytotoxicity on the rat hippocampal neurons at 40 μg/mL, but exhibits no cytotoxic effect on the glial cells[1].

Molecular Weight

1403.62

Formula

C₅₉H₁₀₂N₁₆O₂₁S

CAS No.

144189-71-9

Sequence

Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met

Sequence Shortening

EDVGSNKGAIIGLM

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Cell Assay
[1]

Hippocampal cells are cultured in DMEM supplemented with 10% (v/v) precolostrum newborn calf serum in poly-D-lysine-coated 24-well culture plates at 37°C under a gas mixture of 95% air/5% CO2. After 6 days of culture, non-neuronal cell division is halted by 24 h exposure to 10-5 cytosine arabinoside. After washing cell layers with DMEM, cells are cultured with 1 mL of DMEM per well in the absence of serum for 5 days before experiment. Cells are treated for 3 days with β-Amyloid (22-35) or its amidated (C-terminus) derivative by addition of 20 μL of stock solution (2 mg/mL of either in betaine buffer, pH 8.5, consisting of 0.7% betaine and 20 mM NH4HCO3) to 1.0 mL medium, unless otherwise specified. Control cells are treated with the betaine buffer alone. Morphological change of cells is checked throughout the course of experiment with a phase-contrast microscope. Dead cells are checked by trypan blue staining. Cell injury is assessed by measuring lactate dehydrogenase (LDH) activity released into medium during 3 days of treatment of neurons with peptides[1]
.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

β-Amyloid 22-35Amyloid β-Protein (22-35)Amyloid-ββ-amyloid peptideAbetaInhibitorinhibitorinhibit

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