Aciculatin
Aciculatin is a compound with cytotoxic, anti-inflammatory and anti-arthritic activities, and its synthetic route including specific chemical reaction steps was determined through the first total synthesis.
For research use only. We do not sell to patients.
- CAS No.: 134044-97-6
- Formula: C22H22O8
- Molecular Weight:414.41
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
2.65 μM
Compound: aciculatin
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Cytotoxicity against human CEM cells after 48 hrs by alamar blue assay
Cytotoxicity against human CEM cells after 48 hrs by alamar blue assay
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[PMID: 22272829] |
| HT-29 | IC50 |
25.98 μM
Compound: aciculatin
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Cytotoxicity against human HT-29 cells after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by alamar blue assay
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[PMID: 22272829] |
| MCF7 | IC50 |
3.16 μM
Compound: aciculatin
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Cytotoxicity against human MCF7 cells after 48 hrs by alamar blue assay
Cytotoxicity against human MCF7 cells after 48 hrs by alamar blue assay
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[PMID: 22272829] |
| NCI-H460 | IC50 |
46.94 μM
Compound: aciculatin
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Cytotoxicity against human NCI-H460 cells after 48 hrs by alamar blue assay
Cytotoxicity against human NCI-H460 cells after 48 hrs by alamar blue assay
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[PMID: 22272829] |
Chemical Information
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CAS No. 134044-97-6
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Molecular Weight 414.41
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Formula C22H22O8
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SMILES
COC1=CC(O)=C(C(C=C(C2=CC=C(C=C2)O)O3)=O)C3=C1[C@H]4C[C@@H]([C@@H]([C@H](O4)C)O)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Aciculatin Related Classifications
HY-120529 Related Classifications
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