ALKBH1-IN-1
Based on 1 Customer Validation
ALKBH1-IN-1 (Compound 13h) is a selective ALKBH1 inhibitor, with an IC50 of 0.026 μM and 1.39 μM in the fluorescence polarization and enzyme activity assay, respectively. ALKBH1-IN-1 can modulate the level of DNA 6mA modifications. ALKBH1-IN-1 can be used to study the functions of ALKBH1 and DNA 6mA.
For research use only. We do not sell to patients.
- Purity : 99.63%
- Formula: C16H11F3N4O4
- Molecular Weight:380.28
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All DNA Methyltransferase Isoforms
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Biological Activity
Description
In Vitro
ALKBH1-IN-1 (3 μM, 48 h) stabilizes the ALKBH1 protein in U251 cells overexpressed with ALKBH1[1].
ALKBH1-IN-1 (10 μM, 48 h) increases the amount of 6mA in DNA in U251 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Appearance Solid
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Molecular Weight 380.28
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Formula C16H11F3N4O4
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Color White to off-white
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SMILES
O=C(C1=CN(C2=NC=C(OCC3=CC=CC(OC(F)(F)F)=C3)C=N2)N=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (262.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6296 mL | 13.1482 mL | 26.2964 mL | 65.7410 mL |
| 5 mM | 0.5259 mL | 2.6296 mL | 5.2593 mL | 13.1482 mL | |
| 10 mM | 0.2630 mL | 1.3148 mL | 2.6296 mL | 6.5741 mL | |
| 15 mM | 0.1753 mL | 0.8765 mL | 1.7531 mL | 4.3827 mL | |
| 20 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2871 mL | |
| 25 mM | 0.1052 mL | 0.5259 mL | 1.0519 mL | 2.6296 mL | |
| 30 mM | 0.0877 mL | 0.4383 mL | 0.8765 mL | 2.1914 mL | |
| 40 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6435 mL | |
| 50 mM | 0.0526 mL | 0.2630 mL | 0.5259 mL | 1.3148 mL | |
| 60 mM | 0.0438 mL | 0.2191 mL | 0.4383 mL | 1.0957 mL | |
| 80 mM | 0.0329 mL | 0.1644 mL | 0.3287 mL | 0.8218 mL | |
| 100 mM | 0.0263 mL | 0.1315 mL | 0.2630 mL | 0.6574 mL |
ALKBH1-IN-1 Related Classifications
HY-163385 Related Classifications
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