Anti-CD318/CDCP1 Antibody (25A11)
Anti-CD318/CDCP1 Antibody (25A11) is an anti-CDCP1 antibody. Anti-CD318/CDCP1 Antibody (25A11) drives the internalization of antibody-CDCP1 complexes, inhibits cancer cell migration and invasion, and blocks downstream migration/invasion signaling pathways. When conjugated with saponin, Anti-CD318/CDCP1 Antibody (25A11) mediates the killing of prostate cancer cells. When conjugated with saponin, this antibody acts as an immunotoxin to inhibit the growth and metastasis of primary prostate tumors in mouse xenograft models. Anti-CD318/CDCP1 Antibody (25A11) is applicable to prostate cancer-related research.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Species Reactivity
Human
IC50 & Target
[1]|
CDCP1 |
In Vitro
Anti-CD318/CDCP1 Antibody (25A11) (200 nmol/L) binds to the cell surface of PC-3, DU145, and LNCaP prostate cancer cell lines, confirming CDCP1 protein expression on these cells[1].
Anti-CD318/CDCP1 Antibody (25A11) (2.5 μg/mL) strongly stains PC-3 prostate cancer cells[1].
Anti-CD318/CDCP1 Antibody (25A11) (160 nmol/L; 30 min) binds to a distinct epitope of CDCP1 on PC-3 prostate cancer cells, as prebinding of 25A11 Fab does not inhibit CUB1 antibody binding[1].
Anti-CD318/CDCP1 Antibody (25A11) (0.8 μmol/L; 24 h) potently inhibits PC-3 prostate cancer cell migration (78% inhibition) and invasion (~45% inhibition) in vitro[1].
Anti-CD318/CDCP1 Antibody (25A11, murine and chimeric forms) and its saporin-conjugate (ch25A11-Sap) (0.001-10 nmol/L; 72 h) are internalized by PC-3 prostate cancer cells, leading to dose-dependent cell killing[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC-3 prostate cancer cell lines
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Concentration:0.8 μM
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Incubation Time:24 h
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Result:Inhibited PC-3 cell migration by 78% and PC-3 cell invasion by ~45%, compared to the PBS/complete medium control.
Exhibited migration inhibition superior to the 57% inhibition seen with the positive control 2.5 μmol/L PP2, while the invasion inhibition was comparable to PP2.
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Cell Line:PC-3 prostate cancer cell lines
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Concentration:0.001-10 nM
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Incubation Time:72 h
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Result:Caused dose-dependent PC-3 cell killing, with cell viability decreasing to ~40-45% at the highest tested concentration of 10 nM.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID CB17 (male/female not specified; injected s.c. with 3×106 PC-3 cells on day 0)[1]
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Dosage:0.286 mg/kg (ch25A11); 0.4 mg/kg (ch25A11-Sap)
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Administration:i.v. (days 7, 10, 17; ch25A11 followed by twice weekly s.c. for 3 weeks post primary tumor removal); s.c. (days 7, 10, 17; ch25A11-Sap)
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Result:Did not affect PC-3 primary tumor growth compared to PBS control; showed lymph node metastasis incidence of 7/9 on day 46 and 7/9 on day 50, with a mean metastasis size of 225 mm3 on day 46 and 455 mm3 on day 50 (ch25A11 i.v.).
Inhibited primary tumor growth by 66% on day 18, 67% on day 22, and 63% on day 23; showed lymph node metastasis incidence of 0/8 on day 46 and 1/8 on day 50, with a mean metastasis size of 0 mm3 on day 46 and <4 mm3 on day 50 (ch25A11-Sap i.v.).
Did not inhibit primary tumor growth; showed lymph node metastasis incidence of 0/7 on day 46 and 1/7 on day 50, with a mean metastasis size of 0 mm3 on day 46 and 187 mm3 on day 50 (ch25A11-Sap s.c.).
Gene ID
Accession
Target
CDCP1/CD318
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
ELISA, FACS, Functional assay
Chemical Information
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-CD318/CDCP1 Antibody (25A11) Related Classifications
HY-P992209 Related Classifications
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