Anti-CD4 Antibody (OKT-4)
Based on 1 Customer Validation
Anti-CD4 Antibody (OKT-4) is a kind of mouse IgG2b κ chimeric antibody inhibitor, targeting to human CD4. Anti-CD4 Antibody (OKT-4) can deplete T cells. Anti-CD4 Antibody (OKT-4) can be used for the researches of cancer, infection and immunology, such as melanoma and adenovirus infection.
For research use only. We do not sell to patients.
- Purity: 95.00%
- Molecular Weight:150 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Mouse IgG2b kappa
Human
CD4
Anti-CD4 Antibody (OKT-4) (100 μg, i.p., daily for 3 days) depletes T cells and reduces virus clearance in adenovirus-infected humanized HIS-A2/DR1 mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:MC38/YUMM2.1 tumor f C57BL/6 mice[1]
-
Dosage:250 μg
-
Administration:Intraperitoneally injection, every 2 days
-
Result:Depleted CD4 T cells.
Diminished tumor decrease of anti-PD-1.
-
Animal Model:Adenovirus-infected humanized HIS-A2/DR1 mice (hematopoietic stem cells transplantation)[1]
-
Dosage:100 μg
-
Administration:Intraperitoneally injection, daily for 3 days
-
Result:Depleted T cells.
Abrogated the reduction of virus at day 10.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vitro T cell stimulation/activation; in vivo CD4+ T cell depletion in humanized mice; Flow cyto
-
Flow Cytometry analysis of THP-1 cells labelling CD4 (red) with CD4 Antibody (anti-CD4) (HY-P990861). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃.Mouse IgG2b kappa isotype Control (HY-P99982, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
-
Appearance Liquid
-
Molecular Weight 150 kDa
-
Color Colorless to light yellow
-
SMILES
[Anti-CD4 Antibody (OKT-4)]
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (260 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
[1]. Homet Moreno B, et al. Response to Programmed Cell Death-1 Blockade in a Murine Melanoma Syngeneic Model Requires Costimulation, CD4, and CD8 T Cells. Cancer Immunol Res. 2016 Oct;4(10):845-857. [Content Brief]
[2]. Billerbeck E, et al. Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol. 2013 Aug 15;191(4):1753-64. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)