Anti-CD4 Antibody (OKT-4)
Based on 1 Customer Validation
Anti-CD4 Antibody (OKT-4) is a kind of mouse IgG2b κ chimeric antibody inhibitor, targeting to human CD4. Anti-CD4 Antibody (OKT-4) can deplete T cells. Anti-CD4 Antibody (OKT-4) can be used for the researches of cancer, infection and immunology, such as melanoma and adenovirus infection.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD4
In Vivo
Anti-CD4 Antibody (OKT-4) (100 μg, i.p., daily for 3 days) depletes T cells and reduces virus clearance in adenovirus-infected humanized HIS-A2/DR1 mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MC38/YUMM2.1 tumor f C57BL/6 mice[1]
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Dosage:250 μg
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Administration:Intraperitoneally injection, every 2 days
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Result:Depleted CD4 T cells.
Diminished tumor decrease of anti-PD-1.
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Animal Model:Adenovirus-infected humanized HIS-A2/DR1 mice (hematopoietic stem cells transplantation)[1]
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Dosage:100 μg
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Administration:Intraperitoneally injection, daily for 3 days
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Result:Depleted T cells.
Abrogated the reduction of virus at day 10.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vitro T cell stimulation/activation; in vivo CD4+ T cell depletion in humanized mice; Flow cyto
Verified Bioactivity
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Flow Cytometry analysis of THP-1 cells labelling CD4 (red) with CD4 Antibody (anti-CD4) (HY-P990861). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃.Mouse IgG2b kappa isotype Control (HY-P99982, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-CD4 Antibody (OKT-4)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Homet Moreno B, et al. Response to Programmed Cell Death-1 Blockade in a Murine Melanoma Syngeneic Model Requires Costimulation, CD4, and CD8 T Cells. Cancer Immunol Res. 2016 Oct;4(10):845-857. [Content Brief]
[2]. Billerbeck E, et al. Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol. 2013 Aug 15;191(4):1753-64. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-CD4 Antibody (OKT-4) Related Classifications
HY-P990861 Related Classifications
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