Anti-Mouse CD3ε Antibody (KT3)
Based on 1 Customer Validation
Anti-Mouse CD3ε Antibody (KT3) is a rat-derived IgG2a type antibody inhibitor, targeting to mouse CD3ε. Anti-Mouse CD3ε Antibody (KT3) increases thymocytes proliferation. Anti-Mouse CD3ε Antibody (KT3) can be used for the research of immunology.
For research use only. We do not sell to patients.
- Purity: 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG2a kappa
Mouse
CD3ε
Anti-Mouse CD3ε Antibody (KT3) (72 h) increases T-cell receptor-triggered proliferation in thymocytes[1].
Anti-Mouse CD3ε Antibody (KT3) (1 μg/mL, 30 mins) exhibits impaired Ca2+ fluxes in thymocytes and mature lymph node T cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57B1/10 mice[1]
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Dosage:10 μg
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Administration:Intravenously injection
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Result:Induced preferential death and Fas-L expression in γδ T cells.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
FACS; Research in vivo; in vitro T cell negative selection; in vitro T cell stimulation/activatio
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD3ε (red) with Anti-Mouse CD3ε Antibody (KT3) (HY-P990272). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2a kappa Isotype Control (HY-P990679, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD3ε Antibody (KT3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Hueber AO, et al. Thymocytes in Thy-1-/- mice show augmented TCR signaling and impaired differentiation. Curr Biol. 1997 Sep 1;7(9):705-8. [Content Brief]
[2]. Fischer KD, et al. Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptor. Curr Biol. 1998 May 7;8(10):554-62. [Content Brief]
[3]. Mukasa A, et al. Extensive and preferential Fas/Fas ligand-dependent death of gammadelta T cells following infection with Listeria monocytogenes. Scand J Immunol. 2002 Sep;56(3):233-47. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD3ε Antibody (KT3) Related Classifications
HY-P990272 Related Classifications
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