Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1)
Based on 1 Customer Validation
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) is an anti-mouse E-Cadherin/CD324 IgG1 monoclonal antibody. Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) can downregulate the HER signaling axis and PI3K/Akt/mTOR signaling pathway. Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) can inhibit the proliferation of tumor cells and induce their apoptosis. Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) can be used for researches on cancer and inflammation conditions such as breast cancer, chronic compression injury (CCI) and asthma.
For research use only. We do not sell to patients.
- Purity: 98.53%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Isotype
Rat IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
[1]|
Akt |
mTOR |
HER1 |
HER2 |
HER3 |
HER4 |
In Vitro
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) (20 or 400 µg/mL, 48 h) significantly inhibits cell proliferation and induces apoptosis in MCF-7 and BT474 drug (Trastuzumab) (HY-P9907)-resistant cell line[1].
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) downregulates the HER (HER1, HER2, HER3 and HER4) signaling axis and PI3K/Akt/mTOR signaling pathway (pERK1/2, pAkt, pmTOR, pRaptor and p4E-BP1) in MCF-7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 and BT474-TtzmR cells
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Concentration:20 or 400 µg/mL
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Incubation Time:48 h
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Result:Significantly reduced the number of surviving cells.
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Cell Line:MCF-7 and BT474-TtzmR cells
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Concentration:20 or 400 µg/mL
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Incubation Time:48 h
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Result:Significantly increased the number of apoptotic cells.
In Vivo
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) (1 μg, intrathecal injection, once daily, for 14 days) can reverse the analgesic effect of glial cell-derived neurotrophic factor (GDNF) in male C57BL/6 mice with a chronic compression injury (CCI) model[2].
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) (10 mg/kg, i.p., single dose, 30 min after the second stimulation or 30 min after the first OVA challenge) significantly reduces inflammatory response in male BALB/c mice with a toluene diisocyanate (TDI) or OVA (HY-W250978)/CFA (HY-153808) induced asthma model[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female MMTV-PyMT mice with a spontaneous breast cancer model[1]
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Dosage:1 mg/kg
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Administration:Intraperitoneal injection (i.p.), once a week, from 47 to 90 days of age
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Result:Significantly reduced the number and volume of tumors.
Inhibited the proliferation of tumor cells and induced apoptosis.
Downregulated HER1-4 receptors and their downstream signaling molecules (such as pAkt, pmTOR, survivor).
Was non-toxic to normal tissues.
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Animal Model:Male C57BL/6 mice (20-25 g) with a chronic compression injury (CCI) model caused by surgery[2]
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Dosage:1 μg
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Administration:Intrathecal injection, once daily, for 14 days
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Result:Significantly inhibited the analgesic effect of GDNF.
Blocked the regulation of the E-cadherin/p120ctn signaling pathway by GDNF.
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Animal Model:Toluene diisocyanate (TDI) or OVA/CFA induced male BALB/c mice (6-8 weeks, 20 g)[3]
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Dosage:10 mg/kg
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Administration:Intraperitoneal injection (i.p.), single dose, 30 min after the second stimulation or 30 min after the first OVA challenge
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Result:Significantly reduced sE cadherin levels in bronchoalveolar lavage fluid (BALF).
Reduced inflammatory cell infiltration, airway hyperresponsiveness (AHR), and smooth muscle thickening.
Had no effect on goblet cell metaplasia.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo E-Cadherin neutralization; In vitro E-Cadherin neutralization; Immunofluorescence; Immuno
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Brouxhon SM, et al. Monoclonal antibody against the ectodomain of E-cadherin (DECMA-1) suppresses breast carcinogenesis: involvement of the HER/PI3K/Akt/mTOR and IAP pathways. Clin Cancer Res. 2013 Jun 15;19(12):3234-46. [Content Brief]
[2]. Wang C, et al. Glial cell-derived neurotrophic factor attenuates neuropathic pain in a mouse model of chronic constriction injury: possible involvement of E-cadherin/p120ctn signaling. J Mol Neurosci. 2014;54(2):156-63. [Content Brief]
[3]. Tang H, et al. Soluble E-Cadherin Contributes to Airway Inflammation in Severe Asthma. Am J Respir Cell Mol Biol. 2024 Jul 30. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse E-Cadherin/CD324 Antibody (DECMA-1) Related Classifications
HY-P990248 Related Classifications
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