BACH1 Antibody (YA4463)
(Synonyms: OF; BACH1; FANCJ)
Based on 1 Customer Validation
BACH1 Antibody (YA4463) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BACH1.
-
Host:
Mouse
-
Isotype:
IgG1
-
Application:
IHC-P, FC, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS with 0.05% sodium azide
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
BACH1 Antibody (YA4463) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BACH1.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 105 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 141 kDa
Purified recombinant fragment of human BRIP1 (AA: 904-986) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS with 0.05% sodium azide
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
BACH1 is a DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease. Can unwind RNA:DNA substrates. Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail[1][2][3][4][5][6][7][8].
-
Subcellular Localization
Nucleus; Cytoplasm
-
Expression
Tissue_specificity:It is widely expressed, with the highest expression level in the testes. -
Isoforms & Post-Translational Modification
Q9BX63 has 2 isomers: Q9BX63-1: 140867 Da (predicted); Q9BX63-2: 112466 Da (predicted).
Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated;Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling -
Subunit
Interacts with the replication protein A complex (RPA) via the RPA1 subunit; following DNA damage they colocalize in foci in the nucleus (PubMed:17596542). Binds directly to the BRCT domains of BRCA1 (PubMed:15125843). Interacts with the CIA complex components CIAO1, CIAO2B and MMS19 (PubMed:23585563)
-
SwissProt ID
-
Synonyms
OF; BACH1; FANCJ
Documentation
Powered by Bioz
