CD33 Antibody (YA4715)
(Synonyms: CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33; CD33 nanobody; )CD33 Antibody (YA4715) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD33.
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Host:
Mouse
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Application:
FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in Phosphate-buffered solution.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:5000-100000 | 1-2μg/Test |
Product Details
CD33 Antibody (YA4715) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD33.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
Purified recombinant Human CD33
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in Phosphate-buffered solution.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD33 (Siglec-3) is an important member of the sialic acid-binding immunoglobulin-like lectin family, one of the most classic surface markers of myeloid cells, and one of the most important targets in acute myeloid leukemia (AML). CD33 is an inhibitory immune receptor belonging to the immunoglobulin superfamily (IgSF), and its intracellular structure contains the immunoreceptor tyrosine inhibitory motif (ITIM).
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Peroxisome
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Expression
Tissue_specificity:Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells -
Isoforms & Post-Translational Modification
CD33 has 3 isomers: P20138-1: 39825 Da (predicted); P20138-2: 33890 Da (predicted); P20138-3: 25293 Da (predicted).
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition;Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent -
Subunit
Homodimer; disulfide-linked (PubMed:10887109). Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation (PubMed:10206955, PubMed:10556798).
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SwissProt ID
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Synonyms
CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33; CD33 nanobody;
Documentation
[1]. Vitale C, et al. Engagement of p75/AIRM1 or CD33 inhibits the proliferation of normal or leukemic myeloid cells. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15091-6. [Content Brief]
[2]. Vitale C, et al. Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. [Content Brief]
[3]. Lajaunias F, et al. Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling. Eur J Immunol. 2005 Jan;35(1):243-51. [Content Brief]
[4]. Freeman SD, et al. Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 1995 Apr 15;85(8):2005-12. [Content Brief]
[5]. Paul SP, et al. Myeloid specific human CD33 is an inhibitory receptor with differential ITIM function in recruiting the phosphatases SHP-1 and SHP-2. Blood. 2000 Jul 15;96(2):483-90. [Content Brief]
[6]. Son M, et al. Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression. Sci Rep. 2017 Mar 21;7(1):270. [Content Brief]
[7]. Taylor VC, et al. The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. J Biol Chem. 1999 Apr 23;274(17):11505-12. [Content Brief]
[8]. Ulyanova T, et al. The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 1999 Nov;29(11):3440-9. [Content Brief]
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