CD45RO Antibody (YA4687)

Cat. No.: HY-P84995: Data Sheet User Guide for Antibodies
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CD45RO Antibody (YA4687) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD45RO.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD45RO Antibody (YA4687) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD45RO.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

P08575

Gene ID

5788 [NCBI]

Immunogen

Purified recombinant Human CD45RO

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000
FC FC: Flow Cytometry	1-2μg/Test

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity; (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Synapse

Expression：
Tissue_specificity:Isomer 1: Detected in thymocytes. Isomer 2: Detected in thymocytes. Isomer 3: Detected in thymocytes. Isomer 4: Not detected in thymocytes. Isomer 5: Detected in thymocytes. Isomer 6: Not detected in thymocytes. Isomer 7: Detected in thymocytes. Isomer 8: Not detected in thymocytes.

Isoforms & Post-Translational Modification：P08575 has 8 isomers: P08575-3: 147486 Da (predicted); P08575-4: 131130 Da (predicted); P08575-5: 142623 Da (predicted); P08575-6: 142898 Da (predicted); P08575-7: 140582 Da (predicted); P08575-8: 138034 Da (predicted); P08575-9: 135719 Da (predicted); P08575-10: 135993 Da (predicted).
Heavily N- and O-glycosylated

Subunit：Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1 (PubMed:11909961). Interacts with DPP4; the interaction is enhanced in an interleukin-12-dependent manner in activated lymphocytes (PubMed:12676959)

Synonyms

LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105; CD45RO nanobody;

Documentation

User Guide for Antibodies (1077 KB)