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CD64 Antibody (YA3771)

(Synonyms: FCGR1A; FCRI; CD64A; IGFR1)
Customer Review

Based on 1 Customer Validation

CD64 Antibody (YA3771) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD64.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • CD64 Antibody (YA3771) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CD64 Antibody (YA3771) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD64.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43-80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Purified recombinant fragment of human CD64 (AA: extra 16-145) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌synovial sarcoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌Pancreatic cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌embryonal carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌Endometrial carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    Immunohistochemical analysis of paraffin-embedded human ‌breast cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    FcγRI (CD64) is a high-affinity receptor for the Fc region of immunoglobulin γ. FcγRI (CD64) is constitutively expressed by monocytes, macrophages, and many myeloid progenitor cells. FcγRI (CD64) plays a role in both innate and adaptive immune responses. FcγRI (CD64) mediates the effector function of IgG on monocytes, thereby triggering antibody-dependent cytotoxicity (ADCC) to kill virus-infected cells.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Monocyte/macrophage specific

  • Isoforms & Post-Translational Modification

    CD64 has 2 isomers: P12314-1: 42632 Da (predicted); P12314-2: 38795 Da (predicted).
    Phosphorylated on serine residues

  • Subunit

    Interacts with IGHG1. Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN

  • SwissProt ID

    P12314

  • Gene ID
    2209 [NCBI]
  • Synonyms

    FCGR1A; FCRI; CD64A; IGFR1

[1]. van Vugt MJ, et al. The FcgammaRIa (CD64) ligand binding chain triggers major histocompatibility complex class II antigen presentation independently of its associated FcR gamma-chain. Blood. 1999 Jul 15;94(2):808-17. [Content Brief]

CD64 Antibody (YA3771) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌synovial sarcoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Pancreatic cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌embryonal carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Endometrial carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌breast cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    1/16
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • CD64 Antibody (YA3771) Product Image
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌synovial sarcoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Pancreatic cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌embryonal carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Endometrial carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌breast cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • Experimental Validation Results for CD64 Antibody (YA3771)
  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌synovial sarcoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Pancreatic cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌embryonal carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Endometrial carcinoma tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌breast cancer tissue using CD64 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84074, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD64 Antibody (YA3771) Product Image IHC-P
  • CD64 Antibody (YA3771) Product Image IHC-P
  • CD64 Antibody (YA3771) Product Image IHC-P
  • CD64 Antibody (YA3771) Product Image IHC-P
  • CD64 Antibody (YA3771) Product Image IHC-P
  • CD64 Antibody (YA3771) Product Image IHC-P