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CD69 Antibody (YA4201)

(Synonyms: CLEC2C; CD69)
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CD69 Antibody (YA4201) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD69.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • CD69 Antibody (YA4201) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CD69 Antibody (YA4201) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD69.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 20-30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Immunogen

Purified recombinant fragment of human CD69 aa 69-199.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718918

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    CD69 is a transmembrane protein primarily expressed on mucosa-resident T cells, playing a crucial role in immune cell homeostasis. Following activation by various stimuli-such as antigen recognition or cytokine signaling-CD69 is rapidly expressed on the surface of platelets, T lymphocytes, and NK cells, where it activates distinct signaling pathways across different cell types. CD69 negatively regulates Th17 cell differentiation through a carbohydrate-dependent interaction with galectin-1 (LGALS1) present on immature dendritic cells. It also acts via the S100A8/S100A9 complex on peripheral blood monocytes to promote the conversion of naive CD4+ T cells into regulatory T cells. Mechanistically, it specifically recognizes Pseudomonas aeruginosa, promoting ERK1 activation and the subsequent secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines.

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein

  • Expression


    Tissue_specificity:It is expressed on the surface of activated T cells, B cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets.

    Induction:By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells

  • Subunit

    Homodimer; disulfide-linked. Interacts with S100A8 and S100A9. Interacts with galactin-1/LGALS1. Interacts with S1PR1; this interaction mediates S1PR1 degradation

  • SwissProt ID

    Q07108

  • Gene ID
    969 [NCBI]
  • Synonyms

    CLEC2C; CD69

[1]. de la Fuente H, et al. The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1. Mol Cell Biol. 2014 Jul;34(13):2479-87. [Content Brief]

[2]. Lin CR, et al. Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation. FASEB J. 2015 Dec;29(12):5006-17. [Content Brief]

[3]. Jiménez-Fernández M, et al. CD69-oxLDL ligand engagement induces Programmed Cell Death 1 (PD-1) expression in human CD4 + T lymphocytes. Cell Mol Life Sci. 2022 Aug 5;79(8):468. [Content Brief]

[4]. Jiang Y, et al. CD69 mediates the protective role of adipose tissue-derived mesenchymal stem cells against Pseudomonas aeruginosa pulmonary infection. Clin Transl Med. 2021 Nov;11(11):e563. [Content Brief]

[5]. Chen H, et al. Transmembrane protein CD69 acts as an S1PR1 agonist. Elife. 2023 Apr 11;12:. [Content Brief]

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CD69 Antibody (YA4201) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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