CD80 Antibody (YA10048)
(Synonyms: H-2K(D); H-2 class I histocompatibility antigen K-D alpha chain)CD80 Antibody (YA10048) is a Armenian Hamster-derived and non-conjugated IgG Monoclonal antibody, targeting to CD80.
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Host:
Armenian Hamster
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Isotype:
IgG
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Application:
ICC/IF, FC
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Reactivity :
Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:50-200 | 1:500-2000 |
Product Details
CD80 Antibody (YA10048) is a Armenian Hamster-derived and non-conjugated IgG Monoclonal antibody, targeting to CD80.
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Host Armenian Hamster
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Clonality Monoclonal
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Species ReactivityMouse
CHO cell line transfected with mouse B7 (CD80)
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD80 is a Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (By similarity). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (By similarity). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (By similarity). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses[1][2][3].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies. -
Isoforms & Post-Translational Modification
CD80 has 2 isoforms, Q00609-1: amino acid length is 306, molecular weight is 34590 Da (predicted); Q00609-2: amino acid length is 212, molecular weight is 24051 Da (predicted).
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
H-2K(D); H-2 class I histocompatibility antigen K-D alpha chain
Documentation
[1]. Tseng SY, et al. CD80 cytoplasmic domain controls localization of CD28, CTLA-4, and protein kinase Ctheta in the immunological synapse. J Immunol. 2005 Dec 15;175(12):7829-36. [Content Brief]
[2]. Freeman GJ, et al. Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7. J Exp Med. 1991 Sep 1;174(3):625-31. [Content Brief]
[3]. Good-Jacobson KL, et al. CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation. J Immunol. 2012 May 1;188(9):4217-25. [Content Brief]
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