CHRNA6 Antibody (YA4301)(PBS only)

Cat. No.: HY-P84604A: Data Sheet User Guide for Antibodies
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CHRNA6 Antibody (YA4301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CHRNA6 Antibody (YA4301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA6.

Host

Mouse

Molecular Weight

Predicted band size: 57 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q15825

Gene ID

8973 [NCBI]

Immunogen

Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (Probable). CHRNA6 forms pentameric channels with CHRNB2, CHRNB3 and CHRNA4 that exhibit high sensitivity to ACh and nicotine and are predominantly expressed in only a few brain areas, including dopaminergic neurons, norepirephrine neurons and cells of the visual system (PubMed:16835356). nAChrs containing CHRNA6 subunits mediate endogenous cholinergic modulation of dopamine and gamma-aminobutyric acid (GABA) release in response to nicotine at nerve terminals

Subcellular Localization：Synaptic cell membrane; Multi-pass membrane protein

Isoforms & Post-Translational Modification：Q15825 has 2 isomers: Q15825-1: 56898 Da (predicted); Q15825-2: 55018 Da (predicted).

Subunit：Neuronal AChR is composed of two different types of subunits: alpha and non-alpha (beta). CHRNA6/alpha-6 subunit can be combined to CHRNB2/beta-2, CHRNA4/alpha-4 and CHRNB3/beta-3 to give rise to functional receptors (PubMed:16835356). Heteropentamers containing CHRNB3 have an stoichiometry of (CHRNA6:CHRNB2)2:CHRNB3 (PubMed:16835356). Interacts with LYPD6 (PubMed:27344019)

Synonyms

CHNRA6

Documentation

User Guide for Antibodies (1077 KB)