Dnmt3b Antibody (YA4550)
(Synonyms: ICF; ICF1; M.HsaIIIB)Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 96 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 96 kDa
Purified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Dnmt3b is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Universally present; highly expressed in fetal liver, heart, kidney, and placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isomer 1 is expressed in all tissues except brain, skeletal muscle, and peripheral blood mononuclear cells; isomer 3 is universally present; isomer 4 is expressed in all tissues except brain, skeletal muscle, lung, and prostate; isomer 5 is detected only in the testes, with extremely low expression levels in the brain and prostate. -
Isoforms & Post-Translational Modification
Q9UBC3 has 8 isomers: Q9UBC3-1: 95751 Da (predicted); Q9UBC3-2: 93407 Da (predicted); Q9UBC3-3: 86177 Da (predicted); Q9UBC3-4: 80845 Da (predicted); Q9UBC3-5: 88572 Da (predicted); Q9UBC3-6: 94689 Da (predicted); Q9UBC3-7: 77719 Da (predicted); Q9UBC3-8: 81311 Da (predicted).
Sumoylated;Citrullinated by PADI4 -
Subunit
Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex
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SwissProt ID
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Synonyms
ICF; ICF1; M.HsaIIIB
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