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  4. Monoclonal Antibodies Flow Cytometry Antibodies
  5. Dnmt3b Antibody (YA4550)

Dnmt3b Antibody (YA4550)

Cat. No.: HY-P84853
User Guide for Antibodies Technical Support

Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.

For research use only. We do not sell to patients.

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20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 96 kDa;
Observed band size: 96 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.
Application &
Dilution Ratio
Application Dilution Ratio
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:200-1:1000
FC
FC: Flow Cytometry
1:200-1:400
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Purity affinity purified. Conjugation Non-conjugated
Modification Unmodified Isotype IgG2a
Appearance

Liquid
Formulation

Supplied in PBS with 0.05% sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398)
Subcellular Localization：Nucleus
Expression：
Tissue_specificity:Universally present; highly expressed in fetal liver, heart, kidney, and placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isomer 1 is expressed in all tissues except brain, skeletal muscle, and peripheral blood mononuclear cells; isomer 3 is universally present; isomer 4 is expressed in all tissues except brain, skeletal muscle, lung, and prostate; isomer 5 is detected only in the testes, with extremely low expression levels in the brain and prostate.
Isoforms & Post-Translational Modification：Q9UBC3 has 8 isomers: Q9UBC3-1: 95751 Da (predicted); Q9UBC3-2: 93407 Da (predicted); Q9UBC3-3: 86177 Da (predicted); Q9UBC3-4: 80845 Da (predicted); Q9UBC3-5: 88572 Da (predicted); Q9UBC3-6: 94689 Da (predicted); Q9UBC3-7: 77719 Da (predicted); Q9UBC3-8: 81311 Da (predicted).
Sumoylated;Citrullinated by PADI4
Subunit：Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex
Synonyms
ICF; ICF1; M.HsaIIIB
Documentation
User Guide for Antibodies (1077 KB)

Dnmt3b Antibody (YA4550) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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