FGFR1 Antibody (YA3757)(PBS only)

Cat. No.: HY-P84060A: Data Sheet User Guide for Antibodies
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FGFR1 Antibody (YA3757) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to FGFR1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

FGFR1 Antibody (YA3757) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to FGFR1.

Host

Mouse

분자량

Predicted band size: 92 kDa;

Observed band size: 140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P11362

Gene ID

2260 [NCBI]

Immunogen

Purified recombinant extracellular fragment of human FGFR1 (aa22-376) fused with hIgGFc tag expressed in HEK293 cells.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Nucleus; Cytoplasm, cytosol; Cytoplasmic vesicle

Expression：
Tissue_specificity:This protein was detected in astrocytomas, neuroblastomas, and adrenocortical cell lines. Some subtypes were also detected in foreskin fibroblast cell lines, but subtypes 17, 18, and 19 were not detected in these cells.

Isoforms & Post-Translational Modification：P11362 has 21 isomers: P11362-1: 91868 Da (predicted); P11362-8: 73475 Da (predicted); P11362-17: 6682 Da (predicted); P11362-2: 91668 Da (predicted); P11362-9: 73274 Da (predicted); P11362-3: 82162 Da (predicted); P11362-10: 63769 Da (predicted); P11362-4: 81962 Da (predicted); P11362-11: 63569 Da (predicted); P11362-5: 74133 Da (predicted); P11362-12: 55740 Da (predicted); P11362-6: 73933 Da (predicted); P11362-13: 55540 Da (predicted); P11362-7: 91580 Da (predicted); P11362-14: 81875 Da (predicted); P11362-15: 16487 Da (predicted); P11362-16: 33412 Da (predicted); P11362-18: 33125 Da (predicted); P11362-19: 91760 Da (predicted); P11362-20: 90618 Da (predicted); P11362-21: 95344 Da (predicted).
Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation;Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1;N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus

Subunit：Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro) (PubMed:12181353, PubMed:16597617, PubMed:1697263, PubMed:1722683, PubMed:17623664, PubMed:8663044, PubMed:9655399). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23 (PubMed:19966287). Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains) (PubMed:1379697, PubMed:1656221, PubMed:21765395). Interacts with FRS2 (PubMed:21765395). Interacts with RPS6KA1 (PubMed:15117958). Interacts (via C-terminus) with NEDD4 (via WW3 domain) (PubMed:21765395). Interacts with KL (By similarity). Interacts with SHB (via SH2 domain) (PubMed:12181353). Interacts with GRB10 (PubMed:10454568). Interacts with ANOS1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2 (PubMed:19696444). Interacts with SOX2 and SOX3. Interacts with FLRT1, FLRT2 and FLRT3 (By similarity). Found in a ternary complex with FGF1 and ITGAV:ITGB3 (PubMed:18441324, PubMed:20422052)

Synonyms

CEK; FLG; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; HBGFR

각종 서류

User Guide for Antibodies (1077 KB)