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Glucose-6-phosphate isomerase Antibody (YA3675)

(Synonyms: AMF; NLK; PGI; PHI; GNPI; SA-36; GPI)
Customer Review

Based on 1 Customer Validation

Glucose-6-phosphate isomerase Antibody (YA3675) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucose-6-phosphate isomerase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:10000

Product Details

Description

Glucose-6-phosphate isomerase Antibody (YA3675) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucose-6-phosphate isomerase.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 63 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Immunogen

Purified recombinant fragment of human GPI aa 1-558.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718806

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Western blot analysis of extracts from Rat brain(lane 1(40μg) )
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human pancreas using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    Immunofluorescence analysis of GC-7901 cells labeling G6PI antibody (HY-P83978) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    Glucose-6-phosphate isomerase is an In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Secreted

  • Subunit

    Homodimer; in the catalytically active form (PubMed:11371164, PubMed:12054796, PubMed:12777791). Monomer in the secreted form (PubMed:11371164, PubMed:12054796, PubMed:12777791)

  • SwissProt ID

    P06744

  • Gene ID
    2821 [NCBI]
  • Synonyms

    AMF; NLK; PGI; PHI; GNPI; SA-36; GPI

Glucose-6-phosphate isomerase Antibody (YA3675) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling G6PI antibody (HY-P83978) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    1/16
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain(lane 1(40μg) )
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling G6PI antibody (HY-P83978) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Experimental Validation Results for Glucose-6-phosphate isomerase Antibody (YA3675)
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat brain(lane 1(40μg) )

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Glucose-6-phosphate isomerase Antibody (HY-P83978, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of GC-7901 cells labeling G6PI antibody (HY-P83978) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image WB
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image IHC-P
  • Glucose-6-phosphate isomerase Antibody (YA3675) Product Image ICC