Glucose-6-phosphate isomerase Antibody (YA3675)

Western blot analysis of extracts from Rat brain(lane 1(40μg) ),THP-1(lane 2(40μg) ),Ramos(lane 3(40μg) ),Raji(lane 4(40μg) ),HepG2(lane 5(40μg) ),Raw264.7(lane 6(40μg) ),PC-12(lane 7(40μg) ),COS-7(lane 8(40μg) ),BEL-7402(lane 9(40μg) ),HL-7702(lane 10(40μg) ),SMMC-7721(lane 11(40μg) )and HepG2(lane 12(40μg) ) using G6PI antibody(20198P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Immunofluorescence analysis of GC-7901 cells labeling G6PI antibody (20198P) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with G6PI antibody (20198P) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)