IL-8/CXCL8 Antibody (PBS only)
(Synonyms: Interleukin 8; Interleukin8; (Ala-IL-8)77; (Ser-IL-8)72; 3 10C; 310C; AMCF1; b ENAP; CXC chemokine ligand 8; CXCL 8; CXCL8; Emoctakin; GCP-1; GCP1; IL 8; IL-8; IL-8(1-77); IL-8(9-77); IL8; LYNAP; MDNCF; MDNCF-b; MDNCF-c; MONAP; NAF; NAP-1; NAP1; Protein 3 10C; Protein 3-10C; SCYB 8; SCYB8; TSG1.)IL-8/CXCL8 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-8/CXCL8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF, ELISA
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Reactivity :
Human, Rabbit
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Formulation:
Supplied in PBS.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 | 1:5000-10000 |
Product Details
IL-8/CXCL8 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IL-8/CXCL8.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Rabbit Predicted Reactivity: Pig,Cow,Sheep
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 10 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 8/9 kDa
KLH conjugated synthetic peptide derived from human IL-8: 31-99/99
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IL-8/CXCL8 is a Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways[1][2][3][4][5][6][7][8].
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Subcellular Localization
Secreted
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Expression
Induction: By ER stress in a DDIT3/CHOP-dependent manner. -
Isoforms & Post-Translational Modification
IL-8/CXCL8 has an amino acid length of 99, molecular weight is 11098 Da.
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form -
Subunit
Homodimer.
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SwissProt ID
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Synonyms
Interleukin 8; Interleukin8; (Ala-IL-8)77; (Ser-IL-8)72; 3 10C; 310C; AMCF1; b ENAP; CXC chemokine ligand 8; CXCL 8; CXCL8; Emoctakin; GCP-1; GCP1; IL 8; IL-8; IL-8(1-77); IL-8(9-77); IL8; LYNAP; MDNCF; MDNCF-b; MDNCF-c; MONAP; NAF; NAP-1; NAP1; Protein 3 10C; Protein 3-10C; SCYB 8; SCYB8; TSG1.
Documentation
References
[1]. M Baggiolini, et al. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989 Oct;84(4):1045-9. [Content Brief]
[2]. M Wolf, et al. Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur J Immunol. 1998 Jan;28(1):164-70. [Content Brief]
[3]. Qian Liu, et al. The CXCL8-CXCR1/2 pathways in cancer. Cytokine Growth Factor Rev. 2016 Oct;31:61-71. [Content Brief]
[4]. Jian-Feng Liu, et al. IL-8 Is Upregulated in the Tissue-Derived EVs of Odontogenic Keratocysts. Biomed Res Int. 2022 Jul 30;2022:9453270. [Content Brief]
[5]. Zinkernagel AS, et al. The IL-8 protease SpyCEP/ScpC of group A Streptococcus promotes resistance to neutrophil killing. Cell Host Microbe. 2008 Aug 14;4(2):170-8. [Content Brief]
[6]. Hammond ME, et al. IL-8 induces neutrophil chemotaxis predominantly via type I IL-8 receptors. J Immunol. 1995 Aug 1;155(3):1428-33. [Content Brief]
[7]. Van Damme J, et al. The neutrophil-activating proteins interleukin 8 and beta-thromboglobulin: in vitro and in vivo comparison of NH2-terminally processed forms. Eur J Immunol. 1990 Sep;20(9):2113-8. [Content Brief]
[8]. Jacobi HH, et al. IL-8 and the activation of eosinophils and neutrophils following nasal allergen challenge. Int Arch Allergy Immunol. 1998 May;116(1):53-9. [Content Brief]
[9]. Holmes WE, et al. Structure and functional expression of a human interleukin-8 receptor. Science. 1991 Sep 13;253(5025):1278-80. [Content Brief]
[10]. Murphy PM, et al. Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science. 1991 Sep 13;253(5025):1280-3. [Content Brief]
[11]. Takami M, et al. Signaling pathways involved in IL-8-dependent activation of adhesion through Mac-1. J Immunol. 2002 May 1;168(9):4559-66. [Content Brief]
[12]. Damaj BB, et al. Physical association of Gi2alpha with interleukin-8 receptors. J Biol Chem. 1996 May 31;271(22):12783-9. [Content Brief]
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