Mouse Anti-Human CD45RA Antibody (PE/Cy7)(YA3628)

Cat. No.: HY-P84045M: Data Sheet User Guide for Antibodies
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Mouse Anti-Human CD45RA Antibody (PE/Cy7)(YA3628) is a PE/Cy7-conjugated and Mouse origined IgG2b monoclonal antibody, targeting to CD45RA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Mouse Anti-Human CD45RA Antibody (PE/Cy7)(YA3628) is a PE/Cy7-conjugated and Mouse origined IgG2b monoclonal antibody, targeting to CD45RA.

Host

Mouse

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 147 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P08575

Gene ID

5788 [NCBI]

Immunogen

A synthesized peptide derived from Human PTPRC

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	5μL per test for million cells or whole blood in 100 μL volume.

Purity	Protein A affinity purified.	Conjugation	PE, Cy7
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% Proclin300, 1% BSA.

Storage & Stability

Stored at 2-8°C for 1 year, protect from light, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity; (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Synapse

Expression：
Tissue_specificity:Isomer 1: Detected in thymocytes. Isomer 2: Detected in thymocytes. Isomer 3: Detected in thymocytes. Isomer 4: Not detected in thymocytes. Isomer 5: Detected in thymocytes. Isomer 6: Not detected in thymocytes. Isomer 7: Detected in thymocytes. Isomer 8: Not detected in thymocytes.

Isoforms & Post-Translational Modification：P08575 has 8 isomers: P08575-3: 147486 Da (predicted); P08575-4: 131130 Da (predicted); P08575-5: 142623 Da (predicted); P08575-6: 142898 Da (predicted); P08575-7: 140582 Da (predicted); P08575-8: 138034 Da (predicted); P08575-9: 135719 Da (predicted); P08575-10: 135993 Da (predicted).
Heavily N- and O-glycosylated

Subunit：Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1 (PubMed:11909961). Interacts with DPP4; the interaction is enhanced in an interleukin-12-dependent manner in activated lymphocytes (PubMed:12676959)

Synonyms

PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45

Documentation

User Guide for Antibodies (1077 KB)