Neurofilament Medium Antibody (YA3680)(PBS only)

Cat. No.: HY-P83983A: Data Sheet User Guide for Antibodies
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Neurofilament Medium Antibody (YA3680) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Medium.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Neurofilament Medium Antibody (YA3680) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Medium.

Host

Mouse

Molecular Weight

Predicted band size: 102 kDa;

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P07197

Gene ID

4741 [NCBI]

Immunogen

Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cell projection, axon

Isoforms & Post-Translational Modification：P07197 has 2 isomers: P07197-1: 102472 Da (predicted); P07197-2: 59468 Da (predicted).
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber;Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function;Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

Subunit：Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro) (By similarity). Forms heterodimers with INA (in vitro) (By similarity)

Synonyms

NFM; NEF3; NF-M

Documentation

User Guide for Antibodies (1077 KB)