Nicotinic Acetylcholine Receptor beta 2/CHRNB2 Antibody (YA7101)

Art. -Nr.: HY-P87413: Data Sheet User Guide for Antibodies
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Nicotinic Acetylcholine Receptor beta 2/CHRNB2 Antibody (YA7101) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor beta 2/CHRNB2.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Nicotinic Acetylcholine Receptor beta 2/CHRNB2 Antibody (YA7101) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor beta 2/CHRNB2.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 57kDa;

Species Reactivity

Human

SwissProt ID

P17787

Immunogen

Purified recombinant fragment of human CHRNB2 (AA: extra 26-233) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Reinheit	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). CHRNB2 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3, CHRNA4 and CHRNA6, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed:16835356, PubMed:20881005, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578, PubMed:8663494). Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed:27698419, PubMed:29720657, PubMed:38454578). Heteropentameric channels with CHRNA6 and CHRNA4 exhibit high sensitivity to ACh and nicotine and are predominantly expressed in only a few brain areas, including dopaminergic neurons, norepirephrine neurons and cells of the visual system. nAChrs containing CHRNA6 subunits mediate endogenous cholinergic modulation of dopamine and gamma-aminobutyric acid (GABA) release in response to nicotine at nerve terminals (By similarity). Also forms functional nAChRs with other subunits such as CHRNA7:CHRNB2, mainly expressed in basal forebrain cholinergic neurons (PubMed:33239400, PubMed:38161283)

Subcellular Localization：Synaptic cell membrane,Cell membrane

Isoforms & Post-Translational Modification：P17787: 502 amino acids, molecular weight 57019 Da.

Subunit：Neuronal AChR is a heteropentamer composed of two different types of subunits: alpha and beta

Database

SwissProt: SwissProt: P17787

Dokumentation

User Guide for Antibodies (1077 KB)