NOTCH4 Antibody (YA4442)(PBS only)

Cat. No.: HY-P84745A: Data Sheet User Guide for Antibodies
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NOTCH4 Antibody (YA4442) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NOTCH4.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

NOTCH4 Antibody (YA4442) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NOTCH4.

Host

Mouse

분자량

Predicted band size: 210 kDa;

Observed band size: 230 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99466

Gene ID

4855 [NCBI]

Immunogen

Purified recombinant fragment of human NOTCH4 (AA: extra 118-357) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Nucleus

Expression：
Tissue_specificity:This gene is highly expressed in the heart, moderately expressed in the lungs and placenta, and lowly expressed in the liver, skeletal muscle, kidneys, pancreas, spleen, lymph nodes, thymus, bone marrow, and fetal liver. It is not expressed in adult brain tissue or peripheral blood leukocytes.

Isoforms & Post-Translational Modification：Q99466 has 3 isomers: Q99466-1: 209622 Da (predicted); Q99466-2: 61262 Da (predicted); Q99466-3: 39617 Da (predicted).
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity);Phosphorylated

Subunit：Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4

Synonyms

INT3

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/lb771-hnc-cell.html