PHF7 Antibody (YA8485)
(Synonyms: HSPC045; HSPC226; NYD-SP6)PHF7 Antibody (YA8485) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHF7.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:150-500 | 1:100-250 | 1:100 |
Product Details
PHF7 Antibody (YA8485) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHF7.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 43.6 kDa
Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7 produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PHF7 is an E3 ubiquitin-protein ligase which ubiquitinates histone H3 at 'Lys-14'. Required for male fertility, via inhibition of SPOP-mediated BRDT degradation when in the presence of acetylated histone H4 in early condensing spermatids. Stabilization of BRDT allows it to facilitate histone removal in early condensing spermatids and promote the progression of histone-to-protamine exchange. Promotes the expression of steroidogenesis proteins in the testes, and as a result plays a role in maintaining testosterone levels and repressing osteoclastogenesis. Promotes transcription of cardiac enhancer genes by facilitating binding of cardiac transcription factors such as MEF2C and GATA4 to target gene promoters. Ubiquitinates histone H4. Ubiquitinates histone H2A and H3 as part of the nucleosome core particle[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Highly expressed in Sertoli cells, but not in germ cells in adult testis. Expression in embryonic testis is 30-times lower. Highly expressed in colon, spleen, white blood cells, pancreas, lung, liver, placenta and brain. Detected at lower levels in thymus, small intestine, ovary and kidney -
Isoforms & Post-Translational Modification
Q9BWX1 has two isomers: Q9BWX1-1: 43767 Da (predicted); Q9BWX1-2: 39259 Da (predicted).
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Subunit
Interacts with MEF2C; the interaction promotes MEF2C binding to its transcription targets (By similarity)
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SwissProt ID
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Synonyms
HSPC045; HSPC226; NYD-SP6
Documentation
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