PHF7 Antibody (YA8485)

Cat. No.: HY-P88801: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PHF7 Antibody (YA8485) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHF7.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PHF7 Antibody (YA8485) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHF7.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 43.6 kDa

Species Reactivity

Human

SwissProt ID

Q9BWX1

Immunogen

Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E3 ubiquitin-protein ligase which ubiquitinates histone H3 at 'Lys-14' (By similarity). Required for male fertility, via inhibition of SPOP-mediated BRDT degradation when in the presence of acetylated histone H4 in early condensing spermatids (By similarity). Stabilization of BRDT allows it to facilitate histone removal in early condensing spermatids and promote the progression of histone-to-protamine exchange (By similarity). Promotes the expression of steroidogenesis proteins in the testes, and as a result plays a role in maintaining testosterone levels and repressing osteoclastogenesis (By similarity). Promotes transcription of cardiac enhancer genes by facilitating binding of cardiac transcription factors such as MEF2C and GATA4 to target gene promoters (By similarity). Ubiquitinates histone H4 (PubMed:32726616). Ubiquitinates histone H2A and H3 as part of the nucleosome core particle (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_Specificity: Highly expressed in Sertoli cells, but not in germ cells in adult testis. Expression in embryonic testis is 30-times lower. Highly expressed in colon, spleen, white blood cells, pancreas, lung, liver, placenta and brain. Detected at lower levels in thymus, small intestine, ovary and kidney

Isoforms & Post-Translational Modification：Q9BWX1 has two isomers: Q9BWX1-1: 43767 Da (predicted); Q9BWX1-2: 39259 Da (predicted).

Subunit：Interacts with MEF2C; the interaction promotes MEF2C binding to its transcription targets (By similarity)

Synonyms

HSPC045; HSPC226; NYD-SP6

Documentation

User Guide for Antibodies (1077 KB)