RAD51L1 Antibody (YA8524)(PBS only)

Cat. No.: HY-P88840A: Data Sheet User Guide for Antibodies
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RAD51L1 Antibody (YA8524) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAD51L1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RAD51L1 Antibody (YA8524) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RAD51L1.

Host

Mouse

Molecular Weight

Predicted band size: 42 kDa

Species Reactivity

Human, Mouse

SwissProt ID

O15315

Immunogen

Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway

Subcellular Localization：Nucleus

Expression：
Tissue_Specificity: Expressed in a wide range of tissues

Isoforms & Post-Translational Modification：O15315 has 5 isomers: O15315-3: 42196 Da (predicted); O15315-1: 38257 Da (predicted); O15315-2: 38304 Da (predicted); O15315-4: 40284 Da (predicted); O15315-5: 25527 Da (predicted).
Phosphorylated on tyrosine residues by BCR-ABL

Subunit：Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel (PubMed:11744692, PubMed:11751635, PubMed:11751636, PubMed:11842112, PubMed:11842113, PubMed:12427746, PubMed:14704354)

Synonyms

R51H2; RAD51L1; REC2

Documentation

User Guide for Antibodies (1077 KB)