SERPINB1 Antibody (YA7870)(PBS only)

Cat. No.: HY-P88186A: Data Sheet User Guide for Antibodies
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SERPINB1 Antibody (YA7870) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SERPINB1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SERPINB1 Antibody (YA7870) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SERPINB1.

Host

Mouse

Molecular Weight

Predicted band size: 42.6 kDa

Species Reactivity

Human, Dog, Monkey

SwissProt ID

P30740

Immunogen

Human recombinant protein fragment corresponding to amino acids 77-306 of human SERPINB1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Also acts as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651)

Subcellular Localization：Secreted,Cytoplasm,Cytolytic granule,Early endosome

Expression：
Tissue_Specificity: In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes

Isoforms & Post-Translational Modification：P30740 has two isomers: P30740-1: 42742 Da (predicted); P30740-2: 26073 Da (predicted).

Subunit：Monomer (PubMed:23269243)

Synonyms

EI; ELANH2; HEL-S-27; HEL57; LEI; M/NEI; MNEI; PI-2; PI2

Documentation

User Guide for Antibodies (1077 KB)