TNFRSF25 Antibody (YA4413)

Cat. No.: HY-P84716: Ficha de datos COA
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TNFRSF25 Antibody (YA4413) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TNFRSF25.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

TNFRSF25 Antibody (YA4413) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TNFRSF25.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 45.4 kDa

Species Reactivity

Human

SwissProt ID

Q93038

Gene ID

8718 [NCBI]

Immunogen

Purified recombinant fragment of human TNFRSF25 (AA: extra(25-199)) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

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IHC-P

Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using TNFRSF25 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84716, 1:600 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

Function：Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted; Secreted

Expression：
Tissue_specificity:Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate

Isoforms & Post-Translational Modification：Q93038 has 12 isomers: Q93038-1: 45385 Da (predicted); Q93038-2: 45456 Da (predicted); Q93038-3: 23198 Da (predicted); Q93038-4: 27004 Da (predicted); Q93038-5: 19432 Da (predicted); Q93038-6: 14439 Da (predicted); Q93038-7: 13349 Da (predicted); Q93038-8: 25373 Da (predicted); Q93038-9: 40391 Da (predicted); Q93038-10: 41193 Da (predicted); Q93038-11: 45928 Da (predicted); Q93038-12: 29111 Da (predicted).
(Microbial infection) Glycosylated at Arg-352 by enteropathogenic E.coli protein NleB1;Glycosylated

Subunit：Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4

RRID

AB_3718968

Synonyms

DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; GEF720; WSL-LR; PLEKHG5; TNFRSF12

Documentación

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Ficha de datos (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)