TRIM36 Antibody
(Synonyms: RBCC728, RNF98, TRIM36, E3 ubiquitin-protein ligase TRIM36, RING finger protein 98, RING-type E3 ubiquitin transferase TRIM36, Tripartite motif-containing protein 36, Zinc-binding protein Rbcc728)
Based on 1 Customer Validation
TRIM36 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TRIM36.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-2000 |
Product Details
TRIM36 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TRIM36.
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Host Rabbit
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 90kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 83kDa
A synthesized peptide derived from human TRIM36.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from U-2 OS (lane 1(20μg)) 、U-251MG (lane 2(20μg)) 、Mouse testis (lane 3(20μg)) and Mouse brain (lane 4(20μg)) using TRIM36 Antibody (HY-P811462) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
TRIM36 is an E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins including CTNNB1, FOXA2 and AKT1. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization. Inhibits lung fibroblast proliferation, migration, and differentiation. Mechanistically, mediates 'Lys-48'-linked polyubiquitination of phospho-AKT1, leading to its proteasomal degradation and consequent inhibition of AKT signaling. Promotes apoptosis via inhibition of the Wnt/beta-catenin signaling pathway. Regulates ferroptosis by ubiquitinating and degrading FOXA2[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cytoplasmic vesicle, secretory vesicle, acrosome; Cytoplasm, cytoskeleton
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Expression
Tissue_Specificity: Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart. Expressed in fetal tissues. -
Isoforms & Post-Translational Modification
TRIM36 has 4 isoforms, Q9NQ86-1: amino acid length is 728, molecular weight is 83013 Da (predicted); Q9NQ86-2: amino acid length is 60, molecular weight is 6742 Da (predicted); Q9NQ86-3: amino acid length is 61, molecular weight is 6702 Da (predicted); Q9NQ86-4: amino acid length is 716, molecular weight is 81570 Da (predicted).TRIM36 存在 4 个异构体，Q9NQ86-1：氨基酸个数为 728 个，分子量为 83013 Da (预测)；Q9NQ86-2：氨基酸个数为 60 个，分子量为 6742 Da (预测)；Q9NQ86-3：氨基酸个数为 61 个，分子量为 6702 Da (预测)；Q9NQ86-4：氨基酸个数为 716 个，分子量为 81570 Da (预测)。
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
RBCC728, RNF98, TRIM36, E3 ubiquitin-protein ligase TRIM36, RING finger protein 98, RING-type E3 ubiquitin transferase TRIM36, Tripartite motif-containing protein 36, Zinc-binding protein Rbcc728
Documentation
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