Antitumor agent-82

Cat. No.: HY-151914: Data Sheet Handling Instructions
FAQs
Technical Support

Antitumor agent-82 (compound 6g) is a potent anti-tumor agent. Antitumor agent-82 shows anti-proliferative activity. Antitumor agent-82 induces cell Autophagy by the ATG5/ATG7 signaling pathway.

For research use only. We do not sell to patients.

Antitumor agent-82 Chemical Structure

CAS No. : 3037605-45-8

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC₅₀	2.71 μM Compound: 6g	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
A-375	IC₅₀	11.5 μM Compound: 6g	Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
BGC-823	IC₅₀	24.8 μM Compound: 6g	Antiproliferative activity against human BGC-823 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human BGC-823 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
CWR22R	IC₅₀	3.86 μM Compound: 6g	Antiproliferative activity against human 22Rv1 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human 22Rv1 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
HCT-116	IC₅₀	4.57 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells expressing ATG5-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells expressing ATG5-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	4.71 μM Compound: 6g	Antiproliferative activity against p53 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against p53 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	4.97 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	5.09 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells expressing ATG7-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells expressing ATG7-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	7.36 μM Compound: 6g	Antiproliferative activity against ATG5 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against ATG5 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	7.89 μM Compound: 6g	Antiproliferative activity against ATG7 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against ATG7 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HT-29	IC₅₀	2.02 μM Compound: 6g	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
KB	IC₅₀	8.12 μM Compound: 6g	Antiproliferative activity against human KB cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human KB cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
MCF7	IC₅₀	13.5 μM Compound: 6g	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
SK-OV-3	IC₅₀	10.6 μM Compound: 6g	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]

In Vitro

Antitumor agent-82 (compound 6g) (0-100 μM; 48 h) shows anti-proliferative activity with IC₅₀s of 24.8, 13.5, 11.5, 2.71, 2.02, 4.53 μM for BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells, respectively^[1].
Antitumor agent-82 (0-4 μM; 0-7 days) inhibits cell growth in a dose and time-dependent manner against HCT116 cells^[1].
Antitumor agent-82 (0-5 μM; 0-60 h) activates autophagy by the ATG5/ATG7 signaling pathway in HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells
Concentration:	0-100 µM
Incubation Time:	48 h
Result:	Showed antiproliferative activity with IC₅₀s of 24.8, 13.5, 11.5, 2.71, 2.02, 4.53 µM for BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells, respectively.

Western Blot Analysis^[1]

Cell Line:	HCT116 cells
Concentration:	0-5 µM
Incubation Time:	0-60 h
Result:	Induced autophagy with no influences on the expression of caspase-3, cleaved caspase-3 and p53 protein, significantly increased the expression of LC3-II and p62.

In Vivo

Antitumor agent-82 (45 mg/kg; i.p.; every two days for 16 days) shows anti-cancer activity for mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old male BALB/c mice^[1]
Dosage:	45 mg/kg
Administration:	I.p.; every two days for 16 days
Result:	Reduced tumor volume and resulted in a considerable reduction of the tumor weight of 69.69%.

Molecular Weight

510.72

Formula

C₃₂H₄₂N₆

CAS No.

3037605-45-8

SMILES

CN1CCN(CC1)CC2=NC(CN3CCN(CC3)C)=C4C(C5=C(N4CCCC6=CC=CC=C6)C=CC=C5)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Ao J,et al. Design, synthesis and pharmacological evaluation of β-carboline derivatives as potential antitumor agent via targeting autophagy. Eur J Med Chem. 2022 Nov 26;246:114955. [Content Brief]